Difference between revisions of "Team:Lethbridge/Improve"

Revision as of 01:11, 18 October 2018

The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in E. coli and used for iGEM user applications. Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the Lethbridge iGEM team in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing.

Release Factor 1

Original Part: BBa_K2443029
Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: Release factor 1 (RF1) works with release factor 2 and 3 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.

Original Part: BBa_K2443030

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: Release factor 2 (RF2) works with release factors 1 and 2 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.

Original Part: BBa_K2443004

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief description: Cysteine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443012

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief description: Lysine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443014

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: Phenylalanine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis. The alpha subunit is one of the two subunits for this function.

Original Part: BBa_K2443019

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: Serine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443023

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description:Valine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443024

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: IF1 is a prokaryotic protein that interacts with the ribosome by binding to the A site, preventing aminoacyl tRNA from entering the ribosome.

Original Part: BBa_K2443025

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: IF-3 is a prokaryotic protein that binds to the 30S subunit of the ribosome to increase chances of availability for starting protein synthesis.

Original Part: BBa_K2443027

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description:EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor which catalyzes the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome for the creation of the peptide chain.

Original Part: BBa_K2443028

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: EF-Ts is a prokaryotic elongation factor. EF-Ts acts at the guanine nucleotide exchange factor for EF-Tu. It releases GTP from EF-Tu, enabling EF-Tu to bind to a new GTP molecule and catalyze another tRNA addition.

Original Part: BBa_K2443033

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief Description: Myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP), which is crucial for energy homeostasis in the cell.

Original Part: BBa_K2443035

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief description: NDK is responsible for the exchange of a phosphates between different nucleoside diphosphates (NDP) and triphosphates (NTP).

Original Part: BBa_K2443036

Submitted by: Lethbridge iGEM 2017

Designed by: iGEM17_Lethbridge

Brief description: This protein catalyzes the addition of the proline amino acid onto the peptide chain during protein synthesis.

@@ Line 12: / Line 12: @@
 <div class="content"> <!--everything added for content goes after this line-->
+<div class="oneText-Wrapper">
+<div class="oneText-Text">
+    <p class="f12">The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in <i>E. coli</i> and used for iGEM user applications.  Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the <a href="https://2017.igem.org/Team:Lethbridge">Lethbridge iGEM team </a> in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing. </p>
+</div>
+</div>
-    <p class="f12">The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in <i>E. coli</i> and used for iGEM user applications.  Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the <a href="https://2017.igem.org/Team:Lethbridge">Lethbridge iGEM team </a> in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing.
+<div style="clear: both"></div>
-</p>
-    <h3><a href="http://parts.igem.org/Part:BBa_K2683016" target="_blank">Release Factor 1</a></h3>
+<h3><a href="http://parts.igem.org/Part:BBa_K2683016" target="_blank" class="f12">Release Factor 1</a></h3>
 <img src="https://static.igem.org/mediawiki/2018/9/97/T--Lethbridge--RF1.PNG" alt="RF1 construct">
-     <ul style="list-style-type:none">
+     <ul style="list-style-type:none" class="f11">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443029" target="_blank">BBa_K2443029</a></li>
          <li> Submitted by: Lethbridge iGEM 2017</li>
@@ Line 24: / Line 29: @@
      </ul>
-      <h3><a href="http://parts.igem.org/Part:BBa_K2683017" target="_blank">Release Factor 2</a></h3>
+      <h3><a href="http://parts.igem.org/Part:BBa_K2683017" target="_blank" class="f12>Release Factor 2</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443030" target="_blank">BBa_K2443030</a></li>
@@ Line 33: / Line 38: @@
-       <h3><a href="http://parts.igem.org/Part:BBa_K2683018" target="_blank">Cysteine tRNA Synthetase</a></h3>
+       <h3><a href="http://parts.igem.org/Part:BBa_K2683018" target="_blank" class="f12>Cysteine tRNA Synthetase</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443004" target="_blank">BBa_K2443004</a></li>
@@ Line 41: / Line 46: @@
      </ul>
-         <h3><a href="http://parts.igem.org/Part:BBa_K2683019" target="_blank">Lysine tRNA Synthetase</a></h3>
+         <h3><a href="http://parts.igem.org/Part:BBa_K2683019" target="_blank" class="f12>Lysine tRNA Synthetase</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443012" target="_blank">BBa_K2443012</a></li>
@@ Line 51: / Line 56: @@
-    <h3><a href="http://parts.igem.org/Part:BBa_K2683020" target="_blank">Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) </a></h3>
+    <h3><a href="http://parts.igem.org/Part:BBa_K2683020" target="_blank" class="f12>Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) </a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443014" target="_blank">BBa_K2443014</a></li>
@@ Line 61: / Line 66: @@
-  <h3><a href="http://parts.igem.org/Part:BBa_K2683022" target="_blank">Serine tRNA Synthetase (SerRS) </a></h3>
+  <h3><a href="http://parts.igem.org/Part:BBa_K2683022" target="_blank" class="f12>Serine tRNA Synthetase (SerRS) </a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443019" target="_blank">BBa_K2443019</a></li>
@@ Line 71: / Line 76: @@
-<h3><a href="http://parts.igem.org/Part:BBa_K2683023" target="_blank">Valine tRNA Synthetase (ValRS) </a></h3>
+<h3><a href="http://parts.igem.org/Part:BBa_K2683023" target="_blank" class="f12>Valine tRNA Synthetase (ValRS) </a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443023" target="_blank">BBa_K2443023</a></li>
@@ Line 82: / Line 87: @@
-<h3><a href="http://parts.igem.org/Part:BBa_K2683024" target="_blank">Initiation Factor I (IF1)</a></h3>
+<h3><a href="http://parts.igem.org/Part:BBa_K2683024" target="_blank" class="f12>Initiation Factor I (IF1)</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443024" target="_blank">BBa_K2443024</a></li>
@@ Line 93: / Line 98: @@
-     <h3><a href="http://parts.igem.org/Part:BBa_K2683025" target="_blank">Initiation Factor 3 (IF1)</a></h3>
+     <h3><a href="http://parts.igem.org/Part:BBa_K2683025" target="_blank" class="f12>Initiation Factor 3 (IF1)</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443025" target="_blank">BBa_K2443025</a></li>
@@ Line 104: / Line 109: @@
-     <h3><a href="http://parts.igem.org/Part:BBa_K2683026" target="_blank">Elongation Factor- Thermo Unstable (EF-Tu) </a></h3>
+     <h3><a href="http://parts.igem.org/Part:BBa_K2683026" target="_blank" class="f12>Elongation Factor- Thermo Unstable (EF-Tu) </a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443027" target="_blank">BBa_K2443027</a></li>
@@ Line 114: / Line 119: @@
-  <h3><a href="http://parts.igem.org/Part:BBa_K2683027" target="_blank">Elongation Factor-Thermo Stable (EF-Ts)</a></h3>
+  <h3><a href="http://parts.igem.org/Part:BBa_K2683027" target="_blank" class="f12>Elongation Factor-Thermo Stable (EF-Ts)</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443028" target="_blank">BBa_K2443028</a></li>
@@ Line 125: / Line 130: @@
-  <h3><a href="http://parts.igem.org/Part:BBa_K2683028" target="_blank">MyoKinase (MK)</a></h3>
+  <h3><a href="http://parts.igem.org/Part:BBa_K2683028" target="_blank" class="f12>MyoKinase (MK)</a></h3>
 <img src="https://static.igem.org/mediawiki/2018/c/ce/T--Lethbridge--MK.PNG" alt="MK construct">
      <ul style="list-style-type:none">
@@ Line 137: / Line 142: @@
      </ul>
-  <h3><a href="http://parts.igem.org/Part:BBa_K2683029" target="_blank">Nucleoside Diphosphate Kinase (NDK)</a></h3>
+  <h3><a href="http://parts.igem.org/Part:BBa_K2683029" target="_blank" class="f12>Nucleoside Diphosphate Kinase (NDK)</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443035" target="_blank">BBa_K2443035</a></li>
@@ Line 148: / Line 153: @@
-     <h3><a href="http://parts.igem.org/Part:BBa_K2683030" target="_blank">Peptidyl Prolyl Isomerase (PPiase)</a></h3>
+     <h3><a href="http://parts.igem.org/Part:BBa_K2683030" target="_blank" class="f12>Peptidyl Prolyl Isomerase (PPiase)</a></h3>
      <ul style="list-style-type:none">
          <li>Original Part: <a href="http://parts.igem.org/Part:BBa_K2443036" target="_blank">BBa_K2443036</a></li>

Difference between revisions of "Team:Lethbridge/Improve"

Revision as of 01:11, 18 October 2018

Release Factor 1

Original Part: BBa_K2443030 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief Description: Release factor 2 (RF2) works with release factors 1 and 2 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.

Original Part: BBa_K2443004 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief description: Cysteine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443012 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief description: Lysine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443019 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief Description: Serine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443023 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief Description:Valine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Original Part: BBa_K2443024 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief Description: IF1 is a prokaryotic protein that interacts with the ribosome by binding to the A site, preventing aminoacyl tRNA from entering the ribosome.

Original Part: BBa_K2443025 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief Description: IF-3 is a prokaryotic protein that binds to the 30S subunit of the ribosome to increase chances of availability for starting protein synthesis.

Original Part: BBa_K2443035 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief description: NDK is responsible for the exchange of a phosphates between different nucleoside diphosphates (NDP) and triphosphates (NTP).

Original Part: BBa_K2443036 Submitted by: Lethbridge iGEM 2017 Designed by: iGEM17_Lethbridge Brief description: This protein catalyzes the addition of the proline amino acid onto the peptide chain during protein synthesis.