Difference between revisions of "Team:NCKU Tainan/Description"

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                                 <div id="Bacteria">
 
                                 <div id="Bacteria">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria">
 
                                     <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria">
                                     <p class="pcontent">After doing some research, we cloned some gene fragments  
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                                     <p class="pcontent">After doing some research, we cloned gene fragments  
                                         that can express PRK, CA and Rubisco which is from a unicellular cyanobacteria strain,  
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                                         that can express PRK, CA and Rubisco into <i>E. coli</i>. All of the genes are originated from a unicellular cyanobacteria strain,  
                                         <i>Synchococcus alongatus</i> pcc 7942 and  <i>Synchococcus alongatus</i>  pcc 7002into our model organism, <i>E. coli</i>.  
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                                         <i>Synchococcus alongatus</i> pcc 7942 and  <i>Synchococcus alongatus</i>  pcc 7002.  
 
                                         After cloning three gene fragments into <i>E. coli</i>,  
 
                                         After cloning three gene fragments into <i>E. coli</i>,  
                                         it could build a new metabolic pathway that can accomplish carbon utilization,  
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                                         a new metabolic pathway that can utilize carbon dioxide was established,  
                                         subverting the cognition that only most of the plants could utilize CO<sub>2</sub>.
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                                         subverting the cognition that only autotrophs could utilize CO<sub>2</sub>.
 
                                     </p>
 
                                     </p>
                                     <p class="pcontent">Firstly, <i>E. coli</i> will uptake xylose in the surrounding of its cultivation environment.  
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                                     <p class="pcontent">Initially, <i>E. coli</i> will uptake xylose from the cultivation medium.  
                                         After that, xylose will be metabolized and converted into Ru5P by its own native metabolic pathway.  
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                                         After that, xylose will be metabolized into Ru5P by its own native metabolic pathway.  
                                         Next, PRK catalyzed the convertion from Ru5P to RuBP.  RuBP will react with CO<sub>2</sub>
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                                         Next, PRK catalyzed the phosphorylation of Ru5P to RuBP.  RuBP will be carboxylate into two molecules of 3-PGA. 3-PGA will be converted into a multifunctional intermediate, pyruvate.
                                        ,which functionalized by Rubisco, and then converted into 3-PGA while 3-PGA will become a multifunctional intermediate, pyruvate.
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                                     </p>
 
                                     </p>
 
                                 </div>
 
                                 </div>

Revision as of 03:38, 18 October 2018

Description

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