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<div id="Bacteria"> | <div id="Bacteria"> | ||
<img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria"> | <img class="bigimg" src="https://static.igem.org/mediawiki/2018/5/5e/T--NCKU_Tainan--background_bacteria.png" alt="Bacteria"> | ||
− | <p class="pcontent">After doing some research, we cloned | + | <p class="pcontent">After doing some research, we cloned gene fragments |
− | that can express PRK, CA and Rubisco | + | that can express PRK, CA and Rubisco into <i>E. coli</i>. All of the genes are originated from a unicellular cyanobacteria strain, |
− | <i>Synchococcus alongatus</i> pcc 7942 and <i>Synchococcus alongatus</i> pcc | + | <i>Synchococcus alongatus</i> pcc 7942 and <i>Synchococcus alongatus</i> pcc 7002. |
After cloning three gene fragments into <i>E. coli</i>, | After cloning three gene fragments into <i>E. coli</i>, | ||
− | + | a new metabolic pathway that can utilize carbon dioxide was established, | |
− | subverting the cognition that only | + | subverting the cognition that only autotrophs could utilize CO<sub>2</sub>. |
</p> | </p> | ||
− | <p class="pcontent"> | + | <p class="pcontent">Initially, <i>E. coli</i> will uptake xylose from the cultivation medium. |
− | After that, xylose will be metabolized | + | After that, xylose will be metabolized into Ru5P by its own native metabolic pathway. |
− | Next, PRK catalyzed the | + | Next, PRK catalyzed the phosphorylation of Ru5P to RuBP. RuBP will be carboxylate into two molecules of 3-PGA. 3-PGA will be converted into a multifunctional intermediate, pyruvate. |
− | + | ||
</p> | </p> | ||
</div> | </div> |
Revision as of 03:38, 18 October 2018