Difference between revisions of "Team:TUDelft/Basic Part"
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Revision as of 12:35, 10 September 2018
Basic Parts
When constructing our BioBricks, we made use of the IDT synthesis offer. This way we were able to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. The basic parts forming our fusion protein composite part are documented separately and also exist as separate BioBricks. This is advantageous for those wishing to combine one of our basic parts with a different expression level or compose a different composite part altogether. All BioBricks are submitted with elaborate characterisation so the user may choose the best protein for their project accordingly. Our favourite basic part is dxCas9 BBa_K2643001.
| ♥ | Name | Type | Description | Designer |
|---|---|---|---|---|
| ♥ | BBa_K2643001 | Basic | dxCas9 (HIS Tag) | TU Delft 2018 |
| ♥ | BBa_K2643002 | Basic | Tn5 Transposase (HIS Tag) | TU Delft 2018 |
| BBa_K2643003 | Basic | Glycine Helical peptide Linker (GHL) | TU Delft 2018 | |
| ♥ | BBa_K2643004 | Basic | Coding region (CDS) of human erythropoietin (EPO) hormone | TU Delft 2018 |
| BBa_K2643005 | Basic | Human EPO gene with artificial intron (615 bp) | TU Delft 2018 | |
| BBa_K2643006 | Basic | Human EPO gene with artificial intron (135 bp) | TU Delft 2018 | |
| BBa_K2643007 | Basic | Human EPO gene with 2 artificial introns (615 + 135 bp) | TU Delft 2018 | |
| BBa_K2643008 | Basic | Human EPO gene with mutation 1 (5 bp) | TU Delft 2018 | |
| BBa_K2643009 | Basic | Human EPO gene with mutation 2 (12 bp) | TU Delft 2018 | |
| BBa_K2643010 | Basic | gRNA expression cassette for CRISPR-targeting lacZ | TU Delft 2018 | |
| BBa_K2643011 | Basic | Mosaic Ends-flanked Kanamycin cassette and RFP | TU Delft 2018 |