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+ | <link rel="stylesheet" href="https://2018.igem.org/Template:NCKU_Tainan/css/safety?action=raw&ctype=text/css"> | ||
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+ | <!--Page_Content--> | ||
+ | <div class="container content"> | ||
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+ | <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example"> | ||
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+ | <h1 class="head2">Notebook</h1> | ||
+ | <h3>Construction of Pasr and Pgada</h3> | ||
+ | <p class="pcontent"> | ||
+ | 4/26~4/27</br> | ||
+ | <ul> | ||
+ | <li>PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted | ||
+ | products by DNA gel. </li> | ||
+ | </ul> | ||
+ | 4/28</br> | ||
+ | <ul> | ||
+ | <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and | ||
+ | RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 | ||
+ | alpha and spread the bacteria on LB plates.</li> | ||
+ | </ul> | ||
− | + | 4/30~5/1</br> | |
− | + | <ul> | |
− | <ul> | + | <li>Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel |
− | <li> | + | electrophoresis. Selected the correct colonies for sequencing and cultured them |
− | + | on LB plates.</li> | |
− | + | </ul> | |
− | + | ||
− | </ul> | + | |
− | </ | + | 5/9</br> |
+ | <ul> | ||
+ | <li>Store the correct colonies in glycerol in -80O C.</li> | ||
+ | </ul> | ||
− | < | + | 6/1</br> |
− | < | + | <ul> |
− | < | + | <li>Transformed each plasmid into BL21(DE3)</li> |
− | + | </ul> | |
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− | + | </p> | |
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</html> | </html> | ||
+ | {{NCKU_Tainan/footer}} |
Revision as of 04:59, 22 September 2018