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− | + | <h1 class="head2">Notebook</h1> | |
− | + | <h3>Construction of Pasr and Pgada</h3> | |
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− | 4/30~5/1</br> | + | <p class="pcontent">4/26~4/27</br> </p> |
− | + | <ul> | |
− | + | <li>PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted | |
− | + | products by DNA gel. </li> | |
− | + | </ul></br> | |
− | + | ||
+ | <p class="pcontent">4/28</br> </p> | ||
+ | <ul> | ||
+ | <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and | ||
+ | RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 | ||
+ | alpha and spread the bacteria on LB plates.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">4/30~5/1</br> </p> | ||
+ | <ul> | ||
+ | <li>Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel | ||
+ | electrophoresis. Selected the correct colonies for sequencing and cultured them | ||
+ | on LB plates.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">5/9</br> </p> | ||
+ | <ul> | ||
+ | <li>Store the correct colonies in glycerol in -80O C.</li> | ||
+ | </ul></br> | ||
+ | |||
+ | <p class="pcontent">6/1</br> </p> | ||
+ | <ul> | ||
+ | <li>Transformed each plasmid into BL21(DE3)</li> | ||
+ | </ul></br> | ||
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Revision as of 05:01, 22 September 2018