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− | <div class="col-10"> | + | <div id="sidelist" class="list-Fgroup"> |
− | + | <a class="list-group-item list-group-item-action" href="#list-item-1">Accomplishment</a> | |
+ | <a class="list-group-item list-group-item-action" href="#list-item-2">Introduction</a> | ||
+ | <a class="list-group-item list-group-item-action" href="#list-item-3">Device design</a> | ||
+ | <a class="list-group-item list-group-item-action" href="#list-item-4">Bioreactor</a> | ||
+ | <a class="list-group-item list-group-item-action" href="#list-item-5">Nutrient tank</a> | ||
+ | <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | ||
+ | aria-hidden="true"></i> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-10"> | ||
+ | <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example"> | ||
− | + | <h1 class="head2">Notebook</h1></br></br> | |
− | + | <h3>Construction of Pasr and Pgada</h3></br> | |
− | + | <p class="pcontent">4/26~4/27</br> </p> | |
− | + | <ul> | |
− | + | <li>PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted | |
− | + | products by DNA gel. </li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">4/28</br> </p> | |
− | + | <ul> | |
− | + | <li>Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and | |
− | + | RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5 | |
− | + | alpha and spread the bacteria on LB plates.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">4/30~5/1</br> </p> | |
− | + | <ul> | |
− | + | <li>Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel | |
− | + | electrophoresis. Selected the correct colonies for sequencing and cultured them | |
− | + | on LB plates.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">5/9</br> </p> | |
− | + | <ul> | |
− | + | <li>Store the correct colonies in glycerol in -80O C.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">6/1</br> </p> | |
− | + | <ul> | |
− | + | <li>Transformed each plasmid into BL21(DE3)</li> | |
− | + | </ul></br> | |
− | + | <h3>Construction of Pasr and Pgada</h3></br> | |
− | + | </br></br> | |
− | + | <p class="pcontent">5/9</br> </p> | |
− | + | <ul> | |
− | + | <li>Prepared the M9 medium in different pH value.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">5/11</br> </p> | |
− | + | <ul> | |
− | + | <li>Pre-cultured the DH5 alpha-pSB1C3 -Pasr-sfGFP strain.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">5/12</br> </p> | |
− | + | <ul> | |
− | + | <li>Cultured the DH5 alpha-pSB1C3 -Pasr-sfGFP strain in M9 medium in different pH value. | |
− | + | Measured the OD600 value and fluorescence every hour.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">5/18</br> </p> | |
− | + | <ul> | |
− | + | <li>Pre-cultured DH5 alpha-pSB1C3 –Pgada-RiboJ-sfGFP strain.</li> | |
− | + | <li>Prepared the M9 medium in different pH value.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">5/19</br> </p> | |
− | + | <ul> | |
− | + | <li>Cultured the DH5 alpha-pSB1C3 –Pgada-RiboJ-sfGFP strain in M9 medium in different pH | |
− | + | value. Measured the OD600 value and fluorescence every hour.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">6/2~6/3</br> </p> | |
− | + | <ul> | |
− | + | <li>Repeated the experiment on 5/11~5/12</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">7/29</br> </p> | |
− | + | <ul> | |
− | + | <li>Pre-culture the BL21(DE3)-pSB1C3 –Pasrr-sfGFP strain</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">7/30</br> </p> | |
− | + | <ul> | |
− | + | <li>Cultured the BL21(DE3)-pSB1C3 –Pasr -sfGFP strain on 96 well plate in different pH | |
− | + | value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">9/4</br> </p> | |
− | + | <ul> | |
− | + | <li>Pre-culture the BL21(DE3)-pSB1C3 –Pgada-RiboJ-sfGFP strain</li> | |
− | + | </ul></br> | |
− | + | <p class="pcontent">9/5</br> </p> | |
− | + | <ul> | |
− | + | <li>Cultured the BL21(DE3)-pSB1C3 –Pgada-RiboJ-sfGFP strain strain on 96 well plate in | |
− | + | different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | |
− | + | </ul></br> | |
− | |||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | </div> | ||
</div> | </div> | ||
</body> | </body> |
Revision as of 05:11, 22 September 2018