Oscarliu117 (Talk | contribs) |
Oscarliu117 (Talk | contribs) |
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<a class="list-group-item list-group-item-action" href="#list-item-5">Nutrient tank</a> | <a class="list-group-item list-group-item-action" href="#list-item-5">Nutrient tank</a> | ||
<a class="list-group-item list-group-item-action" href="#list-item-6">pH Meter</a> | <a class="list-group-item list-group-item-action" href="#list-item-6">pH Meter</a> | ||
− | <a class="list-group-item list-group-item-action" href="#list-item-7"> | + | <a class="list-group-item list-group-item-action" href="#list-item-7">pH Meter</a> |
− | <a class="list-group-item list-group-item-action" href="#list-item-8"> | + | <a class="list-group-item list-group-item-action" href="#list-item-8">pH Meter</a> |
− | + | ||
− | + | ||
− | + | ||
<a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x" | ||
aria-hidden="true"></i> | aria-hidden="true"></i> | ||
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<h1 class="head2">Notebook</h1></br></br> | <h1 class="head2">Notebook</h1></br></br> | ||
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+ | |||
+ | <div id="list-item-1"> | ||
<h3>Construction of Pasr and Pgada</h3></br> | <h3>Construction of Pasr and Pgada</h3></br> | ||
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<li>Transformed each plasmid into BL21(DE3)</li> | <li>Transformed each plasmid into BL21(DE3)</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-2"> | |
− | + | ||
<h3>Construction of Pasr and Pgada</h3></br> | <h3>Construction of Pasr and Pgada</h3></br> | ||
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different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | different pH value. Measure the fluorescence every 5 minutes in 30 minutes.</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
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− | + | <div id="list-item-3"> | |
<h3>Construction of CA</h3></br> | <h3>Construction of CA</h3></br> | ||
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<li>Send the plasmid to sequencing</li> | <li>Send the plasmid to sequencing</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-4"> | |
− | + | ||
<h3>Construction of RbcL</h3></br> | <h3>Construction of RbcL</h3></br> | ||
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<li>Sent the gene to sequencing</li> | <li>Sent the gene to sequencing</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-5"> | |
<h3>Construction of RbcXS</h3></br> | <h3>Construction of RbcXS</h3></br> | ||
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<li>Confirmed by DNA gel.</li> | <li>Confirmed by DNA gel.</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-6"> | |
− | + | ||
<h3>Construction of PRK</h3></br> | <h3>Construction of PRK</h3></br> | ||
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sequencing.</li> | sequencing.</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-7"> | |
− | + | ||
<h3>Whole construction</h3></br> | <h3>Whole construction</h3></br> | ||
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<li>Inserted PT7-CA with PLacI-PRK into pSB3K3</li> | <li>Inserted PT7-CA with PLacI-PRK into pSB3K3</li> | ||
</ul></br> | </ul></br> | ||
+ | </div> | ||
− | + | <div id="list-item-8"> | |
<h3>SDS PAGE, Functional test and Total solution</h3></br> | <h3>SDS PAGE, Functional test and Total solution</h3></br> | ||
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<li> Total solution.</li> | <li> Total solution.</li> | ||
</ul></br> | </ul></br> | ||
− | + | </div> | |
Revision as of 05:42, 22 September 2018