Difference between revisions of "Team:NCKU Tainan/Hardware"

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{{NCKU_Tainan/header}} {{NCKU_Tainan/navbar}} {{NCKU_Tainan/style}}
  
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    <link rel="stylesheet" href="https://2018.igem.org/Template:NCKU_Tainan/css/hardware?action=raw&ctype=text/css">
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    <!--Page_Content-->
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    <div class="container content">
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        <h1 class="head">Hardware</h1>
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        <div class="navbar-example">
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            <div class="row">
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                <div class="col-2 side">
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                    <div id="sidelist" class="list-group">
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                        <a class="list-group-item list-group-item-action" href="#list-item-1">Accomplishment</a>
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                        <a class="list-group-item list-group-item-action" href="#list-item-2">Introduction</a>
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                        <a class="list-group-item list-group-item-action" href="#list-item-3">Device design</a>
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                        <a class="list-group-item list-group-item-action" href="#list-item-4">Bioreactor</a>
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                        <a class="list-group-item list-group-item-action" href="#list-item-5">Nutrient tank</a>
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                        <a class="list-group-item list-group-item-action" href="#list-item-6">Materials required</a>
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                        <a class="list-group-item list-group-item-action" href="#"><i class="fa fa-arrow-up fa-1x"
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                                aria-hidden="true"></i></a>
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                    </div>
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                </div>
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                <div class="col-10">
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                    <div data-spy="scroll" data-target="#sidelist" data-offset="0" class="scrollspy-example">
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                        <div class="container">
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                            <div id="list-item-1">
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                                </br></br></br>
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                                <h3>Accomplishment</h3>
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                                <ol>
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                                    <li>Built and characterized a functional prototype for carbon utilization system in
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                                        industry sector.</li>
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                                    <li>Created an instructional video, a manual and lists of materials.</li>
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                                    <li>Implemented Bio-safety to our device.</li>
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                                    <li>Integrated with <a href="https://2018.igem.org/Team:NCKU_Tainan/Model" style="color:#28ff28;">modeling</a>.</li>
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                                    <li>Sense with Arduino.</li>
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                                </ol>
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                            </div>
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                            <div id="list-item-2">
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                                </br></br></br>
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                                <h3>Introduction</h3>
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                                <div id="pt">
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                                    <p class="hpcontent">Nearly 40% of CO2 emissions are attributable to industries.
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                                        The goal of our
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                                        project is to solve the CO2 problem by using engineered E.coli to fix carbon
 +
                                        dioxide emitted from
 +
                                        industries and convert it into bio-product, pyruvate. To accomplish our goal,
 +
                                        we designed a device
 +
                                        that will upscale our project to be used on field and we aim to integrate the
 +
                                        device into
 +
                                        industrial IGCC system. And we used the Arduino to sense the <a href="#list-item-6"
 +
                                            style="color:#28ff28;">pH</a>,
 +
                                        <a href="#list-item-7" style="color:#28ff28;">CO2 concentration</a> and <a href="#list-item-8"
 +
                                            style="color:#28ff28;">temperature</a> then use the <a href="#list-item-9"
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                                            style="color:#28ff28;">wi-fi
 +
                                            sensor</a> to upload to the <a href="https://2018.igem.org/Team:NCKU_Tainan/Software#list-item-3"
 +
                                            style="color:#28ff28;">database</a>.Last but not least, we can monitor the
 +
                                        data by our <a href="https://2018.igem.org/Team:NCKU_Tainan/Software#list-item-4"
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                                            style="color:#28ff28;">app</a>.</p>
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                                </div>
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                            </div>
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                            <div id="list-item-3">
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                                </br></br></br>
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                                <h3>Device design</h3>
 +
                                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">Our device consists of 4 main parts: a bioreactor, a nutrient
 +
                                        tank, a collection
 +
                                        tank and Arduino.The flue gas contains high concentration of CO2 and this will
 +
                                        inhibit the growth
 +
                                        of E.coli. Thus, we reduce CO2 concentration level to less than 5% before
 +
                                        entering the bioreactor
 +
                                        by using flowmeter, which is an instrument for measuring the flow of gases in
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                                        pipelines.</p>
 +
                                </div>
 +
                                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
 +
                                <div id="pt">
 +
                                    <p class="pcontent">For Arduino, we use temperature sensor(DS18B20)、pH meter and
 +
                                        CO2 sensor(MG811) to
 +
                                        monitor our device. Besides, we use LCD to print datum and use Wi-Fi
 +
                                        sensor(ESP8266 Nodemcu) to
 +
                                        upload our records to database as well. (You can see more information about
 +
                                        Arduino code in <a href="https://2018.igem.org/Team:NCKU_Tainan/Software#list-item-1"
 +
                                            style="color:#28ff28;">software</a>.)</p>
 +
                                </div>
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                            </div>
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                            <div id="list-item-4">
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                                </br></br></br>
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                                <h3>Bioreactor</h3>
 +
                                <div id="pt">
 +
                                  <p class="pcontent">We developed a closed bioreactor system and implemented online monitoring system
 +
                                    which can live monitoring several environmental parameters. Here is the detail of our bioreactor.</p>
 +
                                </div>
 +
                                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
 +
                                <div id="pt">
 +
                                  <p class="pcontent">Gas inlet port are located on the bioreactor’s lower part while outlet port are
 +
                                    located on the bioreactor’s upper lid. As low concentration CO2 enters the bioreactor, it flows
 +
                                    through the diffuser refiner and dissolves in the buffered medium to form acid. A pH sensor and
 +
                                    temperature sensor is installed to monitor the bioreactor tank for further control implementation.
 +
                                    Besides, the CO2 concentration level of exhaust gas is monitored by a CO2 gas sensor, which is
 +
                                    mounted on the upper lid. These sensor’s output is connected to an Arduino analog input and sensor
 +
                                    readings are displayed on a serial LCD which is attached on the lid of bioreactor. The data is then
 +
                                    uploaded in real time to a web server via WiFi by using Arduino WiFi Shield.</br></p>
 +
                                </div>
 +
                                </br>
 +
                                <h8>DIY Stirrer</h8></br>
 +
                                <div class="carousel-item active" style="background-image: url('http://placehold.it/1900x1080')"></div>
 +
                                <div id="pt">
 +
                                  <p class="pcontent">To prevent sedimentation of cells at the bottom of bioreactor, we build our own
 +
                                    3D printed slow speed magnetic stirrer which permits gentle mixing of microcarrier cell cultures.
 +
                                    The 3D printed magnet bed is designed specifically for two magnets and can be fitted on the DC
 +
                                    motor. The stirrer works by using a DC motor to spin two magnets with opposite polarity, which
 +
                                    could create a magnetic field in the bioreactor and cause the stir bar to spin and mix the
 +
                                    contents. For controlling the speed of the DC motor, we use Arduino and L298N to control the input
 +
                                    voltage to the motor by using PWM signal.</p>
 +
                                </div>
 +
                              </div>
 +
               
 +
                              <div id="list-item-5">
 +
                                </br></br></br>
 +
                                <h3>Nutrient tank(尚未完成)</h3>
 +
                                <div id="pt">
 +
                                  <p class="pcontent">The nutrients are pumped into the growth chamber at a rate proportional to the
 +
                                    growth factor of the culture, which is determined experimentally through the doubling time of the
 +
                                    particular bacterial strain.</p>
 +
                                </div>
 +
                              </div>
 +
 +
 +
                              <div id="list-item-6">
 +
                                </br></br></br></br>
 +
                                <h3>Materials required</h3>
 +
                                <div id="pt">
 +
                                  <ul>
 +
                                    <li>Acrylic Sheet</li>
 +
                                    <li>Arduino UNO</li>
 +
                                    <li>Power Supply</li>
 +
                                    <li>Batteries</li>
 +
                                    <li>Rotameter</li>
 +
                                    <li>pH meter</li>
 +
                                    <li>Thermometer(DS18B20)</li>
 +
                                    <li>CO2 sensor(MG811)</li>
 +
                                    <li>Wi-fi sensor(ESP8266 NodeMcu)</li>
 +
                                    <li>Geared DC Motor</li>
 +
                                    <li>Tubes</li>
 +
                                    <li>Magnets</li>
 +
                                    <li>3D Printed Structure</li>
 +
                                    <li>Nuts and Screws</li>
 +
                                    <li>Wires</li>
 +
                                    <li>Pumps</li>
 +
                                  </ul>
 +
                                </div>
 +
                              </div>
 +
 +
                        </div>
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{{NCKU_Tainan/footer}}

Revision as of 09:21, 30 September 2018

Hardware




Accomplishment

  1. Built and characterized a functional prototype for carbon utilization system in industry sector.
  2. Created an instructional video, a manual and lists of materials.
  3. Implemented Bio-safety to our device.
  4. Integrated with modeling.
  5. Sense with Arduino.



Introduction

Nearly 40% of CO2 emissions are attributable to industries. The goal of our project is to solve the CO2 problem by using engineered E.coli to fix carbon dioxide emitted from industries and convert it into bio-product, pyruvate. To accomplish our goal, we designed a device that will upscale our project to be used on field and we aim to integrate the device into industrial IGCC system. And we used the Arduino to sense the pH, CO2 concentration and temperature then use the wi-fi sensor to upload to the database.Last but not least, we can monitor the data by our app.




Device design

Our device consists of 4 main parts: a bioreactor, a nutrient tank, a collection tank and Arduino.The flue gas contains high concentration of CO2 and this will inhibit the growth of E.coli. Thus, we reduce CO2 concentration level to less than 5% before entering the bioreactor by using flowmeter, which is an instrument for measuring the flow of gases in pipelines.

For Arduino, we use temperature sensor(DS18B20)、pH meter and CO2 sensor(MG811) to monitor our device. Besides, we use LCD to print datum and use Wi-Fi sensor(ESP8266 Nodemcu) to upload our records to database as well. (You can see more information about Arduino code in software.)




Bioreactor

We developed a closed bioreactor system and implemented online monitoring system which can live monitoring several environmental parameters. Here is the detail of our bioreactor.

Gas inlet port are located on the bioreactor’s lower part while outlet port are located on the bioreactor’s upper lid. As low concentration CO2 enters the bioreactor, it flows through the diffuser refiner and dissolves in the buffered medium to form acid. A pH sensor and temperature sensor is installed to monitor the bioreactor tank for further control implementation. Besides, the CO2 concentration level of exhaust gas is monitored by a CO2 gas sensor, which is mounted on the upper lid. These sensor’s output is connected to an Arduino analog input and sensor readings are displayed on a serial LCD which is attached on the lid of bioreactor. The data is then uploaded in real time to a web server via WiFi by using Arduino WiFi Shield.


DIY Stirrer

To prevent sedimentation of cells at the bottom of bioreactor, we build our own 3D printed slow speed magnetic stirrer which permits gentle mixing of microcarrier cell cultures. The 3D printed magnet bed is designed specifically for two magnets and can be fitted on the DC motor. The stirrer works by using a DC motor to spin two magnets with opposite polarity, which could create a magnetic field in the bioreactor and cause the stir bar to spin and mix the contents. For controlling the speed of the DC motor, we use Arduino and L298N to control the input voltage to the motor by using PWM signal.




Nutrient tank(尚未完成)

The nutrients are pumped into the growth chamber at a rate proportional to the growth factor of the culture, which is determined experimentally through the doubling time of the particular bacterial strain.





Materials required

  • Acrylic Sheet
  • Arduino UNO
  • Power Supply
  • Batteries
  • Rotameter
  • pH meter
  • Thermometer(DS18B20)
  • CO2 sensor(MG811)
  • Wi-fi sensor(ESP8266 NodeMcu)
  • Geared DC Motor
  • Tubes
  • Magnets
  • 3D Printed Structure
  • Nuts and Screws
  • Wires
  • Pumps

Follow us
Contact us

igem.ncku.tainan@gmail.com
No.1, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)