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/*------------Get rid of iGEM stuff----------------------------------*/ | /*------------Get rid of iGEM stuff----------------------------------*/ | ||
Revision as of 20:36, 30 September 2018
CenozoicSupplementary Material
I. Full FASTA Sequences
i. Metallothionein 1 promoter
MT1_promoter_mus_musculus
CCACACGTCACATGGGTCGTCCTATCCGAGCCAGTCGTGCCAAAGGGGCGGTCCCGCTGT
GCACACTGGCGCTCCAGGGAGCTCTGCACTCCGCCCGAAAAGTGCGCTCGGCTCTGCCA
AGGACGCGGGGCGCGTGACTATGCGTGGGCTGGAGCAACCGCCTGCTGGGTGCAAACCC
TTTGCGCCCGGACTCGTCCAACGACTATAAAGAGGGCAGGCTGTCCTCTAAGCGTCACCA
CGACTTCAACGTCCTGAGTACCTTCTCCTCACTTACTCCGTAGCTCCAGCTTCACCAGATCTCGGAA
ii. Metallothionein 2a full promoter
X00504.1 Human metallothionein gene IIA promoter region
ACACGGCGGAGGCGCACGGCGTGGGCACCCAGCACCCGGTACACTGTGTCCTCCCGCTG
CACCCAGCCCCTTCAGCGCGAGGCGTCCCCGAGGCGCAAGTGGGCCGCCTTCAGGGAAC
TGACCGCCCGCGGCCCGTGTGCAGAGCCGGGTGCGCCCGGCCCAGTGCGCGCGGCCGG
GTGTTTCGCTTGGAGCCGCAAGTGACTTCTAGCGCGGGGCGTGTGCAGGGACGGCCGGG
GCGGGGCTTTTGCACTCGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCAC
TCCACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCG
CCTCTTCAGCTCGCCATGATC
iii. Metallothionein 2a minimal promoter version 1
MT2_promoter_59_bp_homo_sapiens
CGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCC
iv. Metallothionein 2a minimal promoter version 2
MT2a_promoter_60_bp_first_A_added_homo_sapiens
ACGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCC
v. Metallothionein 2a minimal promoter version 3
MT2a_promoter_60_bp_last_A_added_homo_sapiens
CGTCCCGGCTCTTTCTAGCTATAAACACTGCTTGCCGCGCTGCACTCCACCACGCCTCCA
vi. CYP1A1 promoter
NG_055251.1:101-1802 Mus musculus Cyp1a1 5' regulatory region (LOC110467519) on chromosome 9
ACTGTCCCCTGCATATCATTTATCTTTCTAGTGCTCTTTTTGTACTATGCCTGTCTCTGTGTT
TTGTTACTTATCACCTGTTTCCCCCATAGCAGCGGGTAAGCTTCGTCAAAGGAGTATTGTTA
ATCCTTTTCACCTCTGCATCAATCACCAGCATCCAGCATAGTAGGAGCTTGGCGAATATTTG
CACAATGCATGAGGGCGGCATGTAACTAGAGTGGGAGGTGAGGGGGTGGATGAAGTGTT
GTTTTTTTTTCCTGGTCTTGGTCACAGAGCAGATATCAATGTTCTTTCTGGGCTCGGGAGCT
CACAGGGGAGGGCAGGTGAAGGTGTTAGTTAGGAACAGGTTGAGTTAGACACGCCAAGTT
CAGATGTTCTTCTTACACTAATCTCACTCTGGGAGCACCCTTCAGGGCCAGAGAGCACCTG
CAAAACAGCCAGCTAGGCGTGCCGGGTGGTGCTGCGTGTCCTGCCACTAAAAATGTGGAC
ACACGCTGTTCAGGCCTTTGCTCTCAGGCAGAAGCCGAGCATCGCACGCAAACCCGGCCC
TTTGCACCGCTGGCGCTGTCTTGTCGCGCCCTTGCAAAGCATAGACTATTTCTATCTCTTAA
ACCCCACCCCAACGCCCCAGGAGAGCTGGCCCTTTAAGAGCCTCACCCACGGTTCCCCTC
CCCCAGCTAGCGTGACAGCACTGGGACCCGCGCCCGGTTGTGAGTTGGGTAGCTGGGTG
GCTGCGCGGGCCTCCAGGCTCTTCTCACGCAACTCCGGGGCACCTTGTCCCCAGCCAGG
TGGGGCGGAGACAGGCAGCCCGACCTCTGCCCCCAGAGGATGGAGCAGGCTTACGCACG
CTAGCCTCAGGAACCTGTGTGCGTGCCAAGCATCACCCTTTGTAGCCCCAGACCCCCTCC
TGCTGTCTCGCGTGGATCCTTCCTCCACCCTTTCCTCCACCATACTTAGATAGCTCTGCAC
CCGCCGCCCGACTTCTCTGTGTTGCCTGCTTCAGTATGTATGCACAACACTAGCAAGCCCC
GGGAGCCTACAGGGAGCTGCAAGCGGGGACTCCCGGGCTTGCAGCTTGCCTAAGGTGAC
CCTTAGAGGTGAGATCTGCAAGCCTGCCATCCATCCCCACCCTCTAGATGAAGCAGCGCG
AACTTCGGCCGATACCCAATTTGTGGGGCACAGAGTCAGTCCAATGGCGCCACTGGCCTT
CCTGTCCTGTGACCTCTGGGCTGGGGTCGTTGCGCTTCTCACGCGAGCTTGGACTCAGTA
ATCCAGGGAAGCAAAGTCACCACCCAGCTGTTCTCCCTCTACCAGCCTTTCCCGGGCACC
CATTGGCTTGTAGTAGGCAAGAGGATCTTACACACTGAATGTTTCAGGGGTGCAAAAATAG
TGAAAAGGAATCCCTATGACCCGGAATGGAGGCCCCAGTACTTACTTTTTAGGTTACCCCA
GAATTTTTCCTCAAACCCCTCCCTCAGTGGGATTATGCACTGTCCATGGAGCACCTTGAAA
GTGTGGGGGGTGGTGACCCCAACCTTTATTCTTTTTCTTTTCTTATGTTTTTTTGTGCCTGTA
CCTACATCAGGTATCCGGTATGGCTTCTTGCCTATCTCCCCCCTCCCCCCTGCCTAGAGCA
CTCCCTAAGGCTGTCCCTCCCTCGGTCCCACCACTGGGCTCAGATAAGGAGGCGTGGCCA
ACAGACACAGAGTCCTATAAAGGTGGTGGTGCCTTCACCCTAACCCTGAAGGTGGTAGTTC
TTGGAGCTTCCCCGATCCTCCCTAGGGTCCTAGAGAACACTCTTCACTTCAGTCCCTCCTT
ACAGCCCAAGGTGAGTGCTTGGGGGGGCTCCTGGATTGCCCCTCCCTCCCTCCCTCCCTC
CCTCCCTCCCTCCCAAGAAAAGGA
Vii. FGF21 promoter
FGF21_promoter_homo_sapiens
GCCACCCCTCCCCCCAGGTTATTTCAGGAGCACCTGCCTGGGCCTGGGATGGCTTCTCTG
GTGAAAGAAACACCAGGATTGCATCAGGGAGGAGGAGGCTGGGATGTCCAGGGTCTGAG
CATCTGAGCAGGGACAGATGAGGTTGAGGTTGGCCCACGGCCAGGTGAGAGGCTTCCAA
GGCAGGATACTTGTGTCTCAGATGCGGTCGCTTCTTTCATACAGCAATTGCCGCCTTGCTG
AGGATCAAGGAACCTCAGTGTCAGATCACGCCCTCCCCCCAAACTTAGAAATTCAGATGGG
GCGCAGAAATTTCTCTTGTTCTGCGTGATCTGCATAGATGGTCCAAGAGGTGGTTTTTCCA
GGAGCCCAGCACCCCTCCTCCCTCCGACTCAGGTGCTTGAGACCCCAGATCCTTCTCTCT
GAGACTCAGGAATGTGGGCCCCCAGCCCCTTTCACCTGGGTCCCAGCTAACCCGATCCTC
CCCTCCCTCATCCCCTAGACCCAGGAGTCTGGCCCTCCATTGAAAGGACCCCAGGTTACA
TCATCCATTCAGGCTGCCCTTGCCACGATGGAATTCTGTAGCTCCTGCCAAATGGGTCAAA
TATCATGGTTCAGGCGCAGGGAGGGTGATTGGGCGGGCCTGTCTGGGTATAAATTCTGGA
GCTTCTGCATCTATCCCAAAAAACAAGGGTGTT
viii. GADD45α promoter
GADD45α_promoter_homo_sapiens
TTGAGAAAGGAGAATCTGGGTTGCCAGGGATTCCCACATGTGGTCACCAGCTCCCCACGC
AGACCAGCTCACGATTTCCCAGTTACACCGGGCAGGTGGGAAACCGTTCTGCTTTCTGTG
GAAAAGATTCTAACTTGGTTCCCTGCCATCCCTGAATACAAACGGGTTGGTTTTTCTTTTTT
GAGCTTCCAACCCTTGCAGCTTTCCAAAAATAAATCAAACCAGCCATCAGGGCACCGAAAT
AATACTACTGCTAATAAGCAGCTTCGCCTAGACTTAGATAAACAACACTTCTGAGGTAAACT
TTGCCCCGGAGGTCTGGAGACACTTTTTTAATGTAACCTGCTTACTAATAATTACTAGACTT
CAGTGCATTAACCCTGGAAATAGATTTTAATAGCCACCCCTTAAAACAAAAGACATGAAAAG
ATAATAAGAAAAAAGTGCCGCAACTATTATAGAAAAACACTTGGCAGCCTGCTTCAGCCCAA
GCTGAGGCCACCTCTAGCCTCTGCTAAAGCCCCCCACTCCCAATGGTCCCCGCCAACCGG
ATAAGAGTGCGCGCGGGACCCGCCTTCCCCTCTCGGCACCGCCCCCGCCCCCGCCCCCT
CGGCTCGCCTCCCGCGTGGCTCCTCCCTTTTCCGCTCCTCTCAACCTGACTCCAGGAGCT
GGGGTCAAATTGCTGGAGCAGGCTGATTTGCATAGCCCAATGGCCAAGCTGCATGCAAAT
GAGGCGGAAGGTGGTTGGCTGAGGGTTGGCAGGATAACCCCGGAGAGCGGGGCCCTTTG
TCCTCCAGTGGCTGGTAGGCAGTGGCTGGGAGGCAGCGGCCCAATTAGTGTCGTGCGGC
CCGTGGCGAGGCGAGGTCCGGGGAGCGAGCGAGCAAGCAAGGCGGGAGGGGTGGCCG
GAGCTGCGGCGGCTGGCACAGGAGGAGGAGCCCGGGCGGGCGAGGGGCGGCCGGAGA
GCGCCAGGGCCTGAGCTGCCGGAGCGGCGCCTGTGAGTGAGTGC
ix. GADD153 promoter
GADD153_promoter_cricetulus_griseus
AGAACAGGAATTCTGGCGTGCAGTGGGCGACTCAGAAACGCCCAAAGGTGCTCCCCCGA
GACAAGCGGTNGGACTACCATGGAAATGCCAGGACCTCAGCCTCTGGGAAGCGTTAGGAG
GTTAAAAGAGATGAGATCCCTTCTAAAGGGCTGGAGAAGATGTCAGTCCAGGTAGGACTAA
TGGAAACTTTATCGCGGTTCCAGGGGCCTCGGNNCGACTGAGCTGGGAGGGGCCGGGAA
GCTGGGAGTCTGGATGGAGGACGAAGTTGGAGGTGATGGGAGGTGGGTGGGCAGACCG
CAGCTCCTGGGCAGACAAGTTCAGGAAGGACAGCCGTTGGGGCCGTTGGATACTGGGAG
CTGGCGCTCCGCCCTCTTCCCTCTCATCCCCCCACCCGCGCCTCCCACCACCGTCGGCG
GCCCCTGCGCGTGCGCGCGCGCAGACACCGGTTGCCAAACATTGCATCATCCCCGCCCC
CCGTCATCCCTCCCTCGCCGCACTCTCCTTCGCCCGCCCGCGCGCGCGCGCGCGCGCGC
GNGCGCATGACTCACTCACCTCCTCCGCGGAGCCTCGTGACCCAAAGCCACTTCCGGGTC
CAAGACAACGTAGCTCTCCAGCCAGAGGGCGGGGCGGAGGCGGGGGCCGAGGGGGCTC
CTGAGTGGCGGATGTAGGGGTGGGGCGGAGTCAGTGCCAGCGTGCCGCTTTCTGATTGG
CAGGCTCCTGGGTCCCGCCCCCCAAAAGAGGGGACGGGCCCGCATAAAATATCTTCTCTC
GGCGCTGCAGAGGTCAGTAGCCTGAGTCTCACACTTCGGTC
II. Plasmid Sequences
i. pcDNA3-EGFP