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+ | <h1>Safety</h1> | ||
+ | <h2>Safety in the lab</h2> | ||
+ | <p> | ||
+ | Security is a prerequisite for any academic research to be ensured. As our team's products are mainly | ||
+ | industry-oriented, safety issues are of the top priority. | ||
+ | Professor.Ding is responsible for the safety and security of our lab and his students will promise the safety | ||
+ | of | ||
+ | daily experiments. | ||
+ | Biosafety officers will guide us how to do a good job in lab safety protection, obey experimental procedures | ||
+ | carefully and operate equipments correctly. It is helpful for us to decrease the risks. | ||
+ | Here are rules in the lab | ||
+ | </p> | ||
+ | <ol> | ||
+ | <li> | ||
+ | Must be familiar with the laboratory and the surrounding environment. | ||
+ | such as water valve, gas valve, switch, safety door location, fire extinguishers and outdoor source | ||
+ | location. | ||
+ | </li> | ||
+ | <li> | ||
+ | When the experiment is carried out, we should not leave the post casually and pay close attention to the | ||
+ | progress of the experiment. | ||
+ | </li> | ||
+ | <li> | ||
+ | In the event of a potentially dangerous experiment, the necessary safety measures, such as wearing | ||
+ | protective glasses, masks or rubber gloves, should be taken in accordance with the experiment. 4. Do | ||
+ | experiments should open doors and windows or ventilation equipment, to maintain indoor air circulation. | ||
+ | Heating volatile harmful liquids, prone to serious odor, easy to pollute the environment in the experiment | ||
+ | should be in the fume hood. | ||
+ | </li> | ||
+ | <li> | ||
+ | Use flammable and explosive chemical reagents, should be away from fire, wear protective glasses, | ||
+ | protective | ||
+ | gloves and so on. | ||
+ | </li> | ||
+ | <li> | ||
+ | Laboratory chemical reagents shall not be imported, smoking or eating in the laboratory is | ||
+ | strictly forbidden. Wash your hands carefully after the experiment is over. Corrosion and irritant drugs, | ||
+ | such | ||
+ | as strong acids, alkalis, glacial acetic acid, and so on, when possible to wear protective glasses, | ||
+ | protective | ||
+ | gloves, dumping, do not straight to the top of the container, when absorbing, should use the ear wash ball. | ||
+ | Disable bare hands to take the above items directly. | ||
+ | </li> | ||
+ | <li> | ||
+ | Beware of electric shocks when using electrical | ||
+ | appliances. Do not use wet hands and objects to contact electrical or electrical plugs when energized. | ||
+ | After the experiment, the electrical power should be cut off. | ||
+ | </li> | ||
+ | <li> | ||
+ | Laboratory staff must be in the laboratory uniform printed and numbered pages of the experimental records | ||
+ | in | ||
+ | detail, the computer stored data can only be used as an attachment, not as a formal record, the | ||
+ | experimental | ||
+ | records must be immediate, objective, detailed, clear, no alteration, tear pages and afterwards to fill the | ||
+ | record; | ||
+ | </li> | ||
+ | <li> | ||
+ | |||
+ | The personnel entering the laboratory shall wear cotton overalls, sandals, high heels or slippers, and | ||
+ | those | ||
+ | with long hair should have their hair pierced, and the work clothes should be replaced when leaving the | ||
+ | laboratory. In the laboratory is strictly forbidden to visitors, noisy. The key of the laboratory is | ||
+ | strictly | ||
+ | forbidden. | ||
+ | </li> | ||
+ | <li> | ||
+ | Each laboratory personnel must be skilled in the use of fire extinguishers. The person who finally leaves | ||
+ | the | ||
+ | laboratory should check the water valve, switch, gas valve and so on, close the door, window, air, | ||
+ | electricity, | ||
+ | gas before leaving the laboratory. | ||
+ | </li> | ||
+ | <li> | ||
+ | To achieve sterilization, open uv lamp for 30 to 60 minutes in sterile room space for 30 to 60 minutes. | ||
+ | </li> | ||
+ | <li> | ||
+ | Check the equipment, must be separately sterilized before moving in sterile room. | ||
+ | </li> | ||
+ | <li> | ||
+ | Pay attention during animal feeding, wear work clothes and gloves, if you are bitten, go to hospital | ||
+ | immediately. | ||
+ | </li> | ||
+ | <li> | ||
+ | Laboratory medicine can not be medical use and shall not be eaten. | ||
+ | </li> | ||
+ | </ol> | ||
+ | |||
+ | <h2>Links of regulation about biosafety.</h2> | ||
+ | <ul> | ||
+ | <li><a href="http://jiuban.moa.gov.cn/fwllm/zxbs/xzxk/bszl/201405/t20140527_3918356.htm">http://jiuban.moa.gov.cn/fwllm/zxbs/xzxk/bszl/201405/t20140527_3918356.htm</a></li> | ||
+ | <li><a href="http://www.zhb.gov.cn/gkml/zj/jl/200910/t20091022_171837.htm">http://www.zhb.gov.cn/gkml/zj/jl/200910/t20091022_171837.htm</a></li> | ||
+ | <li><a href="http://www.gov.cn/gongbao/content/2017/content_5219148.htm">http://www.gov.cn/gongbao/content/2017/content_5219148.htm</a></li> | ||
+ | <li><a href="http://www.gov.cn/gongbao/content/2001/content_60893.htm">http://www.gov.cn/gongbao/content/2001/content_60893.htm</a></li> | ||
+ | </ul> | ||
+ | |||
+ | <h2>Safety of our biological part</h2> | ||
+ | <p> | ||
+ | The experimental materials used in our experiment were not of high risk, such as K-12 E. coli. These materials | ||
+ | are | ||
+ | basically harmless to the human body. | ||
+ | We construct the expression plasmid and its expression of the signaling molecule and the fluorescent protein as | ||
+ | the | ||
+ | natural normal substance, and the expression protein is harmless to the environment. | ||
+ | Our project poses very small risks. The actions we take to reduce those risks include wearing gloves and | ||
+ | uniforms all | ||
+ | the time in the laboratory, removing the gloves after use, do not wear laboratory clothing outside the | ||
+ | laboratory, do | ||
+ | not wear open-toed footwear in the laboratory working areas, do not eat, drink or store food and drink anywhere | ||
+ | in | ||
+ | the laboratory, and sterilizing the waste such as the bacteria culture before discarding them. | ||
+ | </p> | ||
+ | |||
+ | <h2>Safety of the members</h2> | ||
+ | All participants in the experiment were trained in laboratory safety-related training. The following projects are | ||
+ | for | ||
+ | members to receive training programs | ||
+ | <ol> | ||
+ | <li>Lab access and rules (including appropriate clothing, eating and drinking, etc.) </li> | ||
+ | <li>Responsible individuals (such as lab or departmental specialist or institutional biosafety officer) </li> | ||
+ | <li>Differences between biosafety levels</li> | ||
+ | <li>Biosafety equipment (such as biosafety cabinets)</li> | ||
+ | <li>Good microbial technique (such as lab practices)</li> | ||
+ | <li>Disinfection and sterilization</li> | ||
+ | <li>Emergency procedures</li> | ||
+ | <li>Transport rules</li> | ||
+ | <li>Physical biosecurity</li> | ||
+ | <li>.Personnel biosecurity</li> | ||
+ | <li>.Dual-use and experiments of concern</li> | ||
+ | <li>.Data biosecurity</li> | ||
+ | <li>.Chemicals, fire and electrical safety</li> | ||
+ | </ol> | ||
<div class="column full_size"> | <div class="column full_size"> |
Revision as of 09:25, 4 October 2018
Safety
Safety in the lab
Security is a prerequisite for any academic research to be ensured. As our team's products are mainly industry-oriented, safety issues are of the top priority. Professor.Ding is responsible for the safety and security of our lab and his students will promise the safety of daily experiments. Biosafety officers will guide us how to do a good job in lab safety protection, obey experimental procedures carefully and operate equipments correctly. It is helpful for us to decrease the risks. Here are rules in the lab
- Must be familiar with the laboratory and the surrounding environment. such as water valve, gas valve, switch, safety door location, fire extinguishers and outdoor source location.
- When the experiment is carried out, we should not leave the post casually and pay close attention to the progress of the experiment.
- In the event of a potentially dangerous experiment, the necessary safety measures, such as wearing protective glasses, masks or rubber gloves, should be taken in accordance with the experiment. 4. Do experiments should open doors and windows or ventilation equipment, to maintain indoor air circulation. Heating volatile harmful liquids, prone to serious odor, easy to pollute the environment in the experiment should be in the fume hood.
- Use flammable and explosive chemical reagents, should be away from fire, wear protective glasses, protective gloves and so on.
- Laboratory chemical reagents shall not be imported, smoking or eating in the laboratory is strictly forbidden. Wash your hands carefully after the experiment is over. Corrosion and irritant drugs, such as strong acids, alkalis, glacial acetic acid, and so on, when possible to wear protective glasses, protective gloves, dumping, do not straight to the top of the container, when absorbing, should use the ear wash ball. Disable bare hands to take the above items directly.
- Beware of electric shocks when using electrical appliances. Do not use wet hands and objects to contact electrical or electrical plugs when energized. After the experiment, the electrical power should be cut off.
- Laboratory staff must be in the laboratory uniform printed and numbered pages of the experimental records in detail, the computer stored data can only be used as an attachment, not as a formal record, the experimental records must be immediate, objective, detailed, clear, no alteration, tear pages and afterwards to fill the record;
- The personnel entering the laboratory shall wear cotton overalls, sandals, high heels or slippers, and those with long hair should have their hair pierced, and the work clothes should be replaced when leaving the laboratory. In the laboratory is strictly forbidden to visitors, noisy. The key of the laboratory is strictly forbidden.
- Each laboratory personnel must be skilled in the use of fire extinguishers. The person who finally leaves the laboratory should check the water valve, switch, gas valve and so on, close the door, window, air, electricity, gas before leaving the laboratory.
- To achieve sterilization, open uv lamp for 30 to 60 minutes in sterile room space for 30 to 60 minutes.
- Check the equipment, must be separately sterilized before moving in sterile room.
- Pay attention during animal feeding, wear work clothes and gloves, if you are bitten, go to hospital immediately.
- Laboratory medicine can not be medical use and shall not be eaten.
Links of regulation about biosafety.
- http://jiuban.moa.gov.cn/fwllm/zxbs/xzxk/bszl/201405/t20140527_3918356.htm
- http://www.zhb.gov.cn/gkml/zj/jl/200910/t20091022_171837.htm
- http://www.gov.cn/gongbao/content/2017/content_5219148.htm
- http://www.gov.cn/gongbao/content/2001/content_60893.htm
Safety of our biological part
The experimental materials used in our experiment were not of high risk, such as K-12 E. coli. These materials are basically harmless to the human body. We construct the expression plasmid and its expression of the signaling molecule and the fluorescent protein as the natural normal substance, and the expression protein is harmless to the environment. Our project poses very small risks. The actions we take to reduce those risks include wearing gloves and uniforms all the time in the laboratory, removing the gloves after use, do not wear laboratory clothing outside the laboratory, do not wear open-toed footwear in the laboratory working areas, do not eat, drink or store food and drink anywhere in the laboratory, and sterilizing the waste such as the bacteria culture before discarding them.
Safety of the members
All participants in the experiment were trained in laboratory safety-related training. The following projects are for members to receive training programs- Lab access and rules (including appropriate clothing, eating and drinking, etc.)
- Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)
- Differences between biosafety levels
- Biosafety equipment (such as biosafety cabinets)
- Good microbial technique (such as lab practices)
- Disinfection and sterilization
- Emergency procedures
- Transport rules
- Physical biosecurity
- .Personnel biosecurity
- .Dual-use and experiments of concern
- .Data biosecurity
- .Chemicals, fire and electrical safety
Safety
Please visit the Safety Hub to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.
On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)
Safe Project Design
Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:
- Choosing a non-pathogenic chassis
- Choosing parts that will not harm humans / animals / plants
- Substituting safer materials for dangerous materials in a proof-of-concept experiment
- Including an "induced lethality" or "kill-switch" device
Safe Lab Work
What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!
Safe Shipment
Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?