While running our course in iGEM, despite the help and mentorship of our instructors, we were left with challenges and difficulties
that only people in our shoes can understand. By this mean, we were in touch with many teams via social network. We communicated
with teams to help each other out in overcoming the hardships together and met brilliant people. Thus, the collaborations with the
other iGEM teams: “LACAS BioBots, Warwick, Saint Joseph, iGEM Dusseldorf, iGEM Marburg, iGEM UPF-CRG Barcelona, iGEM Vilnius-Lithuania,
iGEM Michigan State University, iGEM Paris Bettencourt, iGEM Oxford, iGEM Oxford, iGEM Imperial, Bilkent UNAMBG, Acıbadem Istanbul,
Bielefeld” which are mentioned below in a more detailed way emphasize our joy and efforts spent during the exchange of ideas and knowledge.
LACAS BioBots 2018 iGEM
We helped the team iGEM Lahore, LACAS BioBots from Pakistan via a Skype session we had in which we discussed about the ongoing
status of our projects and the InterLab processes. About the Interlab process; we gave them suggestions based on the difficulties
we faced and shared our own experiences as we were ahead of them during that time. We gave them the key points of some protocols
as we had setbacks due to misunderstandings. Moreover, we wrote a paragraph based on art and synthetic biology for a competition
they were organizing worldwide.
Warwick 2018 iGEM
With Warwick iGEM 2018 team we had several Skype sessions where we discussed about our projects, experiments and methods.
They introduced us to websites such as benchling, where the FASTA(s) of desired gene(s) can be written and so the gene(s) are
virtually inserted to a certain backbone (such as pSB1C3). The site enables the user to see any possible cut sites, which primers
can be and should be used and your gene circuit on the whole.
The Warwick team, furthermore answered our questions via Instagram and provided help when we faced some problems with our protocols.
Saint Joseph iGEM 2018
While exchanging knowledge with Saint Joseph Istanbul iGEM Team from Turkey, we talked about our projects and since it’s their first
year experiencing iGEM, we gave them advice mostly on how to characterize a part. We also helped them in the means of medal criteria and
how to finish their work on time; we suggested them to start filling both their wiki page and pages in the registry. In order for them
to interact with us any time they are in need, we exchanged our phone numbers and kept in contact at all times.
Surveys To Greater Populations...
For the iGEM Copenhagen 2018 and iGEM Paris Bettencourt, we filled their surveys in order to help them with their projects. The teams
iGEM Dusseldorf, iGEM Marburg, iGEM UPF-CRG Barcelona, iGEM Vilnius-Lithuania, iGEM Michigan State University, iGEM Paris Bettencourt,
iGEM Oxford helped us spread our survey to greater populations. We supported iGEM Oxford and iGEM Imperial teams by spreading their
survey as well.
iGEM 2018 Turkey Meet-up
With the attendance of all iGEM 2018 Teams from Turkey, we held a meet-up in our school's conference center on the 8th of September, 2018.
Our goal in this meet-up was to get to know iGEM teams from Turkey, to gain helpful new insights, polish everyone's presentation skills,
and to exchange advice, tips and thoughts.
Since it's the first time that another high school iGEM Team from Turkey will be participating iGEM this year, we were especially enthusiastic
to have Saint Joseph HS iGEM Team as a guest, and share our experiences with them.
We had the meet-up opening with the sincere music performance of students from Mavi Çizgi music school. After the music, Tuğba İnanç ,our PI,
talked about her experiences in iGEM with previous teams and officially initiated presentation session. Her presentation was followed by
Dr. Ceyhun Kayıhan's speech in which he talked about the history of Synthetic Biology and its impact on the society.
Then, team presentations started and followed the order as Saint Joseph iGEM, Bilkent UNAMBG, METU_HS_Ankara, and finally Acıbadem İstanbul.
We all have asked questions to each other, gave advice and shared our memories from the summer we left behind. It was refreshing to see new
interpretations and ways of doing things. Also it was motivating to see that we were not the only ones to face problems and spend efforts to
overcome them.
After the scientific presentations session, to have some fun and build stronger bonds as iGEMmers, together with Saint Joseph team, we followed
the route to a tour in METU campus, which is known to be green, refreshing and fun. Along the way, we shared our hilarious memories and struggles
throughout our journey of iGEM.
We all think that this meetup was productive, and we wish all the teams the best of luck.
Modeling With Bielefeld
As a highschool team, we were short on knowledge such as programming and modelling. Another setback was working through the summer, when many
people were on vacation, but the iGEM teams.
During the meetup presentations, one of the teams mentioned getting help and support from the iGEM Bielefeld CeBiTec 2018 team. They also
mentioned being glad with how their project turned out. So we reached out to them.
Through our chats on Instagram, they acknowledged using multiple modeling approaches; they modeled toxicity kinetics of different metal ions
and promoters which they can use for metal uptake related parts and a filter system for water purification. Moreover, they also had a software
tool for siRNA, which can be used for modeling. Unfortunately none of these modelling approaches suited our project in any aspect. However,
they wanted to help us improve our project, thus, they found us several programs that we could use to make a 3D model of an enzyme.
They sent us a list of tutorials and steps necessary to have a proper model. Additionally they taught us how to calculate the binding energy
between two proteins and then to load the proteins into the software tool; Chimera. Here we could modify them by removing helixes and use them
for further calculations in online software tools, like Flexpepdock, to calculate binding strength.