Difference between revisions of "Team:Macquarie Australia/Notebook"
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| − | + | We went through lab safety induction to ensure safety procedures were followed at all times in the lab<br> | |
| − | We went through lab safety induction to ensure safety procedures were followed at all times in the lab | + | <br> |
| − | < | + | We learnt how to perform digestion, ligation, transformation and how to make and run agarose gels<br> |
| − | + | <br> | |
| − | < | + | We used digestion to screen the Photosystem II (PSII) parts DCA, psbELJTB and psbMZtlwkOPQR. psbELJTB and psbMZtlwkOPQR showed successful digestion and correct sizes. DCA showed signs of contamination in all samples.<br> |
| − | We learnt how to perform digestion, ligation, transformation and how to make and run agarose gels | + | <br> |
| − | < | + | We decided to focus of the Chlorophyll plasmid rather than PS II. We proceeded with digestion and ligation of ChlD with ChlI2 as well as DRV1 with ChlG, both into Kanamycin backbones, following the 3A assembly protocol.<br> |
| − | + | <br> | |
| − | < | + | Renee, Areti and Karl also began the interlab study: |
| − | We used digestion to screen the Photosystem II (PSII) parts DCA, psbELJTB and psbMZtlwkOPQR. psbELJTB and psbMZtlwkOPQR showed successful digestion and correct sizes. DCA showed signs of contamination in all samples. | + | |
| − | < | + | |
| − | + | ||
| − | < | + | |
| − | We decided to focus of the Chlorophyll plasmid rather than PS II. We proceeded with digestion and ligation of ChlD with ChlI2 as well as DRV1 with ChlG, both into Kanamycin backbones, following the 3A assembly protocol. | + | |
| − | < | + | |
| − | + | ||
Revision as of 14:07, 6 October 2018
Project planning - no wet lab work this week.
We went through lab safety induction to ensure safety procedures were followed at all times in the lab
We learnt how to perform digestion, ligation, transformation and how to make and run agarose gels
We used digestion to screen the Photosystem II (PSII) parts DCA, psbELJTB and psbMZtlwkOPQR. psbELJTB and psbMZtlwkOPQR showed successful digestion and correct sizes. DCA showed signs of contamination in all samples.
We decided to focus of the Chlorophyll plasmid rather than PS II. We proceeded with digestion and ligation of ChlD with ChlI2 as well as DRV1 with ChlG, both into Kanamycin backbones, following the 3A assembly protocol.
Renee, Areti and Karl also began the interlab study:
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