Difference between revisions of "Team:NCTU Formosa/Composite Part"

We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.

The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.

Bacteriocins

Subtilosin-Intein-CBD(BBa_K2599008)

Bovicin HJ50-Intein-CBD(BBa_K2599009)

Enterocin A-Intein-CBD(BBa_K2599010)

Enterocin B-Intein-CBD(BBa_K2599011)

Enterocin 96-Intein-CBD(BBa_K2599012)

Lacticin Z-Intein-CBD(BBa_K2599013)

Leucocyclicin Q-Intein-CBD(BBa_K2599014)

Durancin TW-49M-Intein-CBD(BBa_K2599015)

Improvement

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      .text{
-       font-size: 3.5vmin;
+       font-size: 4vmin;
        width: 50%;
        position: relative;
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    <div class="wrapper">
      <div class="banner">
-       <img class="cover" src="https://static.igem.org/mediawiki/2018/e/ed/T--NCTU_Formosa--Parts.png">
+       <img class="cover" src="https://static.igem.org/mediawiki/2018/6/63/T--NCTU_Formosa--Parts2.0.png">
      </div>
      <div class="sec1">
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 <!--------------------------------------------------------------------->
        <div class="basic_parts">
-         <div class="title_1"><p>Antimicrobial Peptide</p></div>
+         <div class="title_1"><p>Bacteriocins</p></div>
          <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599008" class="name" target="_blank" style="text-decoration: none;"><p>Subtilosin-Intein-CBD(BBa_K2599008)</p></a>
          <img src="https://static.igem.org/mediawiki/2018/b/bf/T--NCTU_Formosa--Sub_2.png" class="bac">