Difference between revisions of "Team:Toulouse-INSA-UPS/Basic Part"

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<h1 class="heavy">Basic Parts</h1>
 
<h1 class="heavy">Basic Parts</h1>
  
<hr/>
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    <h2 class="heavy">Parts deposed on the registry</h2>
<h3>Sirius</h3>
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        <h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/>
 +
        <figure class="figure" align= middle>
 +
                <img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/4/4f/T--Toulouse-INSA-UPS--Parts--Youn--Cerberus.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 +
                <figcaption class="figure-caption"><strong>Figure 2 :</strong> Part BBa_K2668010</figcaption>
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            </figure>
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        <p>
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                This part consists on a CBM3a fused with mSA2, an engeneered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containgin a UAG codon on his C terminus linker for amber suppression unnatural amino acid incorporation.
 +
                <br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668010" target="_blank">page</a>.
 +
        </p>
 +
        <br/>
 +
<h3>Sirius: One head made of the Cellulose-Binding Modules of Type 3a (CBM3a) fused to mRFP1</h3>
 
<hr/>
 
<hr/>
 
<figure class="figure" align= middle>
 
<figure class="figure" align= middle>
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</p>
 
</p>
 
<br/>
 
<br/>
<h3>Cerberus Coli</h3><hr/>
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<figure class="figure" align= middle>
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<h3>mTagBFP AviTag</h3><hr/>
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/4/4f/T--Toulouse-INSA-UPS--Parts--Youn--Cerberus.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
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<figcaption class="figure-caption"><strong>Figure 2 :</strong> Part BBa_K2668010</figcaption>
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</figure>
+
<p>
+
This part consists on a CBM3a fused with mSA2, an engeneered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containgin a UAG codon on his C terminus linker for amber suppression unnatural amino acid incorporation.
+
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668010" target="_blank">page</a>.
+
</p>
+
<br/>
+
<h3>BFP AviTag</h3><hr/>
+
 
<figure class="figure" align= middle>
 
<figure class="figure" align= middle>
 
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/d/dc/T--Toulouse-INSA-UPS--Parts--Youn--BFP.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">
 
<img style="width : 70%; heigth = auto;" src="https://static.igem.org/mediawiki/2018/d/dc/T--Toulouse-INSA-UPS--Parts--Youn--BFP.PNG" class="figure-img img-fluid rounded" alt="A generic square placeholder image with rounded corners in a figure.">

Revision as of 22:51, 9 October 2018

Basic Parts

Parts deposed on the registry

Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)


A generic square placeholder image with rounded corners in a figure.
Figure 2 : Part BBa_K2668010

This part consists on a CBM3a fused with mSA2, an engeneered version of Streptavidin and Rhizavidin, on his N terminus endogenous linker and containgin a UAG codon on his C terminus linker for amber suppression unnatural amino acid incorporation.
For more details about the design, refer to the corresponding registry page.


Sirius: One head made of the Cellulose-Binding Modules of Type 3a (CBM3a) fused to mRFP1


A generic square placeholder image with rounded corners in a figure.
Figure 1 : Part BBa_K2668020

This part results of the fusion of mRFP1 to CBM3a. The aim of this construct is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept
For more details about the design, refer to the corresponding registry page.


mTagBFP AviTag


A generic square placeholder image with rounded corners in a figure.
Figure 3 : Part BBa_K2668070

mTag Blue Fluorescent Protein fused with an AviTag for in vivo biotinylation by BirA.
For more details about the design, refer to the corresponding registry page.