Difference between revisions of "Team:British Columbia/Interlab"

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              <a href="#">Kent CHEN</a>
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           <h1>HUMAN PRACTICES SILVER</h1>
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             This year, the theme of our Human Practices endeavors was Altering public perception of genetic engineering. In addition to acquiring input from established academics and leaders in industry regarding the laboratory component of the project, we decided to write an investigative journalism piece on the attitudes people have towards science, and host workshops for high school students by partnering with Geering Up, a local non-profit organization.
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             The purpose of the 2018 iGEM Interlab study is to establish a standardized GFP measurement protocol that can reduce the variability between different labs performing the same experiments. It is known that when we take measurements with a plate reader to measure OD600, there can be large variability in these measurements due to the difference in the numbers of cells in the sample.
  
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To best convey what we gained from our interviews, we adapted and used the AREA framework as described by the <a href="https://2017.igem.org/Team:Exeter/HP/Silver"> University of Exeter</a>:
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This year, the proposed question was, “Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?”
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<br><strong>Anticipate</strong>: What we hoped to gain from the interview</center>
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The official protocol for the Interlab study can be found on the official iGEM Interlab Study page here. Our data for the Interlab Study is as follows:
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<br><strong>Engage</strong>: Overview of what was discussed during the interview</center>
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<br><strong>Act</strong>: Our next steps that were influenced by the interview</center
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Revision as of 02:15, 15 October 2018

INTERLAB

OVERVIEW

The purpose of the 2018 iGEM Interlab study is to establish a standardized GFP measurement protocol that can reduce the variability between different labs performing the same experiments. It is known that when we take measurements with a plate reader to measure OD600, there can be large variability in these measurements due to the difference in the numbers of cells in the sample. This year, the proposed question was, “Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?” The official protocol for the Interlab study can be found on the official iGEM Interlab Study page here. Our data for the Interlab Study is as follows:

WHAT

Experts in journalism, marketing, and psychology were interviewed in order to investigate why people hold certain attitudes towards science and the external factors that shape them. These interviews served as keystones for our final article.

WHY

Investigating public perception of science is nothing new, but we wanted to dive deeper and analyze the reasons behind views held by people. We believed that only by studying underlying reasons can we develop innovative methods to alter beliefs.

OUTCOME

[Link to our actual article - to be written lol]

GEERING UP WORKSHOPS

WHAT

In August, we partnered with Geering Up, a local non-profit organization that hosts STEM camps for students. Our workshops were directed at high school students, during which they learned about genetic engineering and grew bacteria on LB-agar plates.

WHY

We worked with Geering Up because we believe their values - making STEM education inclusive and accessible - aligns with ours. Furthermore, we valued the opportunity to introduce high school students to the field of genetic engineering.