Difference between revisions of "Team:NEU China B/Collaborations"
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After the meeting, they also give us some help about our experiment. As our project is closely related to lactic acid fermentation, students from South China Agricultural University have provided us with yogurt for the detection of lactic acid content, providing samples for our Experiments. | After the meeting, they also give us some help about our experiment. As our project is closely related to lactic acid fermentation, students from South China Agricultural University have provided us with yogurt for the detection of lactic acid content, providing samples for our Experiments. | ||
</p> | </p> | ||
| + | |||
| + | <h1> | ||
| + | Collaboration with DUOT | ||
| + | </h1> | ||
| + | <p> | ||
| + | Our two teams have held a meeting to discuss some question about the whole project. | ||
| + | During the meeting, we solved some problems of our projects. | ||
| + | </p> | ||
| + | <div> | ||
| + | Question from NEU | ||
| + | <br> | ||
| + | Quiz1：The idea of your team is very novel and has important practical value. But as a medicine that enters the human body, how to control it does not harm the intestinal flora? | ||
| + | <br> | ||
| + | Answer: We have reviewed a group quantity control system of Imperial College of Science and Technology in 2016. We have made improvements in light of our own situation. Simply put, the concentration of signal substances controlled by the bacteria itself controls the opening and closing of the suicide system, thereby regulating the number of bacteria. | ||
| + | <br> | ||
| + | Quiz2：Your final product is a bacterial preparation. There are great potential threats to the environment. Have you thought about any suicide mechanism? We are doing a cold lethal system, and if you are interested we can cooperate. | ||
| + | <br> | ||
| + | Answer：Thank you very much for your proposal. We also checked the information and found the Blu-ray lethal system, etc., but I am still very happy to work with you. You can provide our plasmids. We can help you to characterize and provide relevant data. | ||
| + | <br> | ||
| + | Question from DUOT | ||
| + | <br> | ||
| + | Quiz1: As a system for detecting lactic acid, what are the advantages of your products with lactic acid detection products on the market? | ||
| + | <br> | ||
| + | Answer: Compared with the normal detection of lactic acid products, we have more use of biological related technologies. By referring to some of the design experience of the 2015-year team at the ETH Zurich, we used the group sensing system of E. coli and used fiber optic circuitry for detection,. It is low cost and ease of use. | ||
| + | <br> | ||
| + | Quiz2: How do you ensure that E. coli in your system does not pollute the fermentation industry? | ||
| + | <br> | ||
| + | Answer: We intend to paste our product into a portable device, and the part that touches the fermentation liquid is only the probe. The E. coli inside the unit does not pollute the fermentation broth. | ||
Revision as of 02:52, 15 October 2018
Collaboration with Fujian normal university
NEU:
Our two teams focused on the problems encountered in the experiment, including some enzymes that we did not use like Gibson enzymes. Our team mainly raised the problem of the actual operation of importing two plasmids into bacteria. In response to this problem, the students of Fujian Normal University gave us a solution.
（photos about our meeting)
We have two teams in the construction of the plasmid also carried out a certain level of cooperation. The students of Fujian Normal University helped us to characterize plasmid p-LsrA-GFP. We helped them to measure the fluorescence value of proU-GFP and construct the plasmid. (http://parts.igem.org/Part:BBa_K2570020)
They tell us that we need to import the first plasmid and then the second plasmid. In addition, they also provide us with some experience about fermentation industry.
Collaboration with SCAU
Our team and the iGEM team of South China Agricultural University conducted an exchange discussion at South China Agricultural University in August 2018. This meeting is mainly to share the two Team's project main content and two teams each encountered in the experiment Problem.
In the meeting, the students of South China Agricultural University show us some their Mathematical models, and we also discuss some problems about our experiments.
Q1: How to match the concentration of AI-2 expressed by the first plasmid pCFDuet-1 with the GFP concentration expressed by pet28a.
A: Our experiment was to release the AI-2 signal molecule after the PCFDUET-1 expression plasmid induction of lactic acid concentration, and AI-2 signal molecule can inhibit the lldr sequence on the second plasmid pet28a.
The LLDR sequence is capable of producing GFP-deterring proteins. In the experiment, we verified that two plasmid copies were more similar, and that the O1o2 sequence could be started normally when the lactic acid concentration was lower (less than 1m mol), and then the GFP gene could be produced fluorescent signal.
Therefore, the expression of two plasmids is more matched, and the experimental results are not affected in this project.
Q2: How do you ensure that your transformant plasmids are not lost?
A: We used the cyanobacterial efficient shuttle plasmid pfq20 given to us by the Wuhan Aquatic Institute of China. We inserted the target gene into the middle of the homologous arm slr0168 at both ends of pfq20. The homology arm can integrate our target fragment into our cyanobacterial genome, allowing our inserted genes to be stably expressed in cyanobacteria, so there is no problem of plasmid loss.
After the meeting, they also give us some help about our experiment. As our project is closely related to lactic acid fermentation, students from South China Agricultural University have provided us with yogurt for the detection of lactic acid content, providing samples for our Experiments.
Collaboration with DUOT
Our two teams have held a meeting to discuss some question about the whole project. During the meeting, we solved some problems of our projects.
Quiz1：The idea of your team is very novel and has important practical value. But as a medicine that enters the human body, how to control it does not harm the intestinal flora?
Answer: We have reviewed a group quantity control system of Imperial College of Science and Technology in 2016. We have made improvements in light of our own situation. Simply put, the concentration of signal substances controlled by the bacteria itself controls the opening and closing of the suicide system, thereby regulating the number of bacteria.
Quiz2：Your final product is a bacterial preparation. There are great potential threats to the environment. Have you thought about any suicide mechanism? We are doing a cold lethal system, and if you are interested we can cooperate.
Answer：Thank you very much for your proposal. We also checked the information and found the Blu-ray lethal system, etc., but I am still very happy to work with you. You can provide our plasmids. We can help you to characterize and provide relevant data.
Question from DUOT
Quiz1: As a system for detecting lactic acid, what are the advantages of your products with lactic acid detection products on the market?
Answer: Compared with the normal detection of lactic acid products, we have more use of biological related technologies. By referring to some of the design experience of the 2015-year team at the ETH Zurich, we used the group sensing system of E. coli and used fiber optic circuitry for detection,. It is low cost and ease of use.
Quiz2: How do you ensure that E. coli in your system does not pollute the fermentation industry?
Answer: We intend to paste our product into a portable device, and the part that touches the fermentation liquid is only the probe. The E. coli inside the unit does not pollute the fermentation broth.