We tried to express the eight antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
Difference between revisions of "Team:NCTU Formosa/Composite Part"
| Line 95: | Line 95: | ||
.bac { | .bac { | ||
| + | width: 40%; | ||
| + | margin: 50px; | ||
| + | margin-left: 30%; | ||
| + | margin-bottom: 70px; | ||
| + | } | ||
| + | |||
| + | .curcumin { | ||
width: 40%; | width: 40%; | ||
margin: 50px; | margin: 50px; | ||
| Line 184: | Line 191: | ||
<div class="basic_parts"> | <div class="basic_parts"> | ||
<div class="title_1"><p>Bacteriocins</p></div> | <div class="title_1"><p>Bacteriocins</p></div> | ||
| − | |||
| − | |||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599009" class="name" target="_blank" style="text-decoration: none;"><p>Bovicin HJ50-Intein-CBD(BBa_K2599009)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--NCTU_Formosa--Bov_2.png" class="bac"> | ||
| − | |||
| − | |||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599011" class="name" target="_blank" style="text-decoration: none;"><p>Enterocin B-Intein-CBD(BBa_K2599011)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> | <img src="https://static.igem.org/mediawiki/2018/5/5f/T--NCTU_Formosa--Ent_B_2.png" class="bac"> | ||
| Line 200: | Line 203: | ||
<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> | <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2599015" class="name" target="_blank" style="text-decoration: none;"><p>Durancin TW-49M-Intein-CBD(BBa_K2599015)</p></a> | ||
<img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > | <img src="https://static.igem.org/mediawiki/2018/b/b4/T--NCTU_Formosa--Dur_2.png" class="bac" style="margin-bottom: 50px;" > | ||
| + | |||
| + | <div class="title_1"><p>Curcumin Bio-sensor</p></div> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K2599017" class="name" target="_blank" style="text-decoration: none;"><p>GS Linker-αS1 Casein(BBa_K2599017)</p></a> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/9/90/T--NCTU_Formosa--%CE%B1S1-casein.png" class="curcumin" style="margin-bottom: 50px;"> | ||
<div class="title_1"><p>Improvement</p></div> | <div class="title_1"><p>Improvement</p></div> | ||
| Line 219: | Line 226: | ||
{{NCTU_Formosa/Footer}} | {{NCTU_Formosa/Footer}} | ||
| + | {{NCTU_Formosa/Top_button}} | ||
Revision as of 08:14, 15 October 2018
Bacteriocins
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Curcumin Bio-sensor
GS Linker-αS1 Casein(BBa_K2599017)
Improvement
