Difference between revisions of "Team:SCAU-China/ProjectOverview"

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       <p>There are many reasons for land desertification, and one of the most important factors is water. By increasing the moisture content of the soil, it will contribute to the growth of the plant and thus slow down the desertification process.
 
       <p>There are many reasons for land desertification, and one of the most important factors is water. By increasing the moisture content of the soil, it will contribute to the growth of the plant and thus slow down the desertification process.
 
Because cellulose has a lot of hydroxyl groups and can bind to a lot of water molecules, our project is to improve soil moisture retention by using cellulose produced in genetically modified organisms.
 
Because cellulose has a lot of hydroxyl groups and can bind to a lot of water molecules, our project is to improve soil moisture retention by using cellulose produced in genetically modified organisms.
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       <p>There are many different types and structures of cellulose. After comparing and searching the data, we decided to use bacterial cellulose (from Acetobacter xylinum) as the target product.
 
       <p>There are many different types and structures of cellulose. After comparing and searching the data, we decided to use bacterial cellulose (from Acetobacter xylinum) as the target product.
 
The bacterial cell synthesis-related gene bcs operon has a fairly high diversity. After analysis, we selected the seven most suitable genes：
 
The bacterial cell synthesis-related gene bcs operon has a fairly high diversity. After analysis, we selected the seven most suitable genes：
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       <p>We successfully obtained two transformed individuals of cyanobacteria and tested them for cellulose content.
 
       <p>We successfully obtained two transformed individuals of cyanobacteria and tested them for cellulose content.
 
But we have a problem: the cellulose content has increased to 180%, and the growth rate has been suppressed.
 
But we have a problem: the cellulose content has increased to 180%, and the growth rate has been suppressed.
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<p>The chassis that we used belongs to the P1 level according to the classification of biosafety.</p>
 
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      <p>1. We use E.coli strains termed DH5alpha and DH10B for the molecular cloning. They are non-pathogenic and have been widely used for gene cloning in many labs around the world. It has been shown that they are barely toxic to researchers when handled properly. We have participated in the biological safety course provided by our university and been well trained for handling the E.coli safely.
 
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2.In addition, Acetobacter xylinum and cyanobacteria (Synechocystis sp., Scytonema javanicum and Microcoleus vaginatus) are safe microorganisms. and they can't live without of the culture medium and do not have any harmful effects on human health.
 
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<p>3. Finally, all organisms are sterilized prior to being discarded.
 
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       <p>School's name:SCAU</p>
 
       <p>School's name:SCAU</p>

Revision as of 07:44, 16 October 2018

SCAU-2018
overview
Background

There are many reasons for land desertification, and one of the most important factors is water. By increasing the moisture content of the soil, it will contribute to the growth of the plant and thus slow down the desertification process. Because cellulose has a lot of hydroxyl groups and can bind to a lot of water molecules, our project is to improve soil moisture retention by using cellulose produced in genetically modified organisms. >>>>>>READ MORE BACKGROUND.

Design

There are many different types and structures of cellulose. After comparing and searching the data, we decided to use bacterial cellulose (from Acetobacter xylinum) as the target product. The bacterial cell synthesis-related gene bcs operon has a fairly high diversity. After analysis, we selected the seven most suitable genes： bcsA,bcsB,bcsC,bcsD,bcsZ,bcsX and bcsH. The benthic organism we chose was Microcoleus vaginatus. So we designed a homologous recombination kit to transfer BC genes of interest into Microcoleus vaginatus by homologous recombination.At the same time, our kit can also be used to convert a variety of different cyanobacteria. For the convenience of later modeling, we used this kit to simultaneously transform the model organism ( Synechocystis sp. pcc6803).

>>>>>>READ MORE ABOUT DESIGN OR HOMOLOGOUS RECOMBINATION KIT.

Result

We successfully obtained two transformed individuals of cyanobacteria and tested them for cellulose content. But we have a problem: the cellulose content has increased to 180%, and the growth rate has been suppressed. We have designed two different scenarios for this in two different ways:

1.in vivo (Mathematical Model of Biological Intrinsic Regulation System)

2.in vitro (Method for Optimizing Microbial Cell Culture).

For the Mathematical Model of Biological Intrinsic Regulation System, we also designed a cas9 gene knockout kit. >>>>>>READ MORE ABOUT RESULT OR MATHEMATICAL MODEL OF BIOLOGICAL INTRINSIC REGULATION SYSTEM OR METHOD FOR OPTIMIZING MICROBIAL CELL CULTURE OR CAS9 GENE KNOCK KIT. )

Tools list

School's name:SCAU

Member's name:SCAU

Designed by:SCAU