Difference between revisions of "Team:Newcastle/Protocols"

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Revision as of 16:21, 16 October 2018

Alternative Roots/Protocols

Experiments

Protocols

Overview
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EXPERIMENTS

PROTOCOLS

Protocol Author Category PDF File
Glycerol Stock Preserves Connor Trotter General View
Bacterial Isolation from Freeze-Dried Pellet Connor Trotter General View
Cell Counting Connor Trotter Chemotaxis View
Minimal A Salt Agar Chemotaxis Assay Connor Trotter Chemotaxis View
Growth Curve in Liquid Culture Sadiya Quazi Chemotaxis View
Planting Seeds in Agar-filled Microcentrifuge tubes Lewis Tomlinson Endophyte Development View
Preparation and Staining of Microscopy samples Lewis Tomlinson Endophyte Development View
Planting Seeds in Agar-filled pipette-tip racks Lewis Tomlinson Endophyte Development View
Re-isolating endophytes Lewis Tomlinson Endophyte Development View
Electroporation of Pseudomonas sp. Frank Eardley Endophyte Development View
MIC Opentrons Code (2 Plates) Frank Eardley Endophyte Development View
Amplification of gblocks and gradient PCR Heather Bottomley Naringenin Operon Biosynthesis View
Colony PCR of transformed DH5α cells Heather Bottomley Naringenin Operon Biosynthesis View
DPN1 digestion and purification Heather Bottomley Naringenin Operon Biosynthesis View
Gel extraction and purification of the gblocks Heather Bottomley Naringenin Operon Biosynthesis View
Gibson Assembly of gblocks and pSB1C3 backbone Heather Bottomley Naringenin Operon Biosynthesis View
Making chemically competent cells Heather Bottomley Naringenin Operon Biosynthesis View
Miniprep of transformed colonies Heather Bottomley Naringenin Operon Biosynthesis View
Amplification, Purification and Quantification of the pSB1C3 backbone by PCR Heather Bottomley Naringenin Operon Biosynthesis View
Quantifying DNA concentration Heather Bottomley Naringenin Operon Biosynthesis View
Resuspending gblocks and primers Heather Bottomley Naringenin Operon Biosynthesis View
SOC media preparation Heather Bottomley Naringenin Operon Biosynthesis View
Transformation of the gblocks into pSB1C3 Heather Bottomley Naringenin Operon Biosynthesis View