Overview
In order to achieve portable and simple field allergy detection, we designed and manufactured the hardware testing equipment for this project - a small, inexpensive ($17) system, including a device can measure OD600 and fluorescence intensity, and a small automatic vibration device avoiding the sinking of cells.
The device supports battery or micro USB for power supply. According to the prompts on the display, you can get started quickly; the device will judge your allergies based on the cell concentration and fluorescence intensity after a certain period of reaction.
In addition to the detection applied to this project, this device can also be used in other environments where fast and portable spectrophotometric detection or fluorescence intensity detection is needed.
We refer to the design of Team Aachen, 2014, but our Devices are more automated and miniaturized.
Designed steps for usage
- Activate the yeast.
- Mix the blood and allergen, turn on our measure device (click the power button on the left).
- After the time on the display is completed (or press the GO button to skip the timing, if you have another timer), add the serum (filter with a syringe and filter to obtain serum / if it is a hospital scene, the blood drawn from the vein can be centrifuged) and t yeast solution to the cuvette; cover with waterproof breathable membrane.
- Open the lid, place the cuvette in the test hole, then close the lid, and press ‘GO’.
- When the display prompts, stick the vibrating device to the cuvette and insert the battery, press ‘GO’.
- When the oscillation time is up, remove the motor, then close the cover and press ‘GO’.
- Wait for the result to appear on the display.
- Turn off the power (double-click the power button). Dispose of waste.
Performance testing
OD Testing
It can be seen that the data of our device is basically consistent with that of spectrophotometer in the laboratory. So our device is also able to reflect the cell concentration.
Design and components
In order to make such a device, we have to minimize the size when we select of the components and design the bracket.
The overall design
Our reaction system is about 800μL, which heights approximately 8mm in the common 10mm cuvette. Therefore, we choose the position which optical pass is 4mm high. Although simply changing filter paper can reuse photosensor, to make it more automatically, we use two photosensors with different light filters to sense different light. Optical pass design is displayed in figure. If we switch on LED in a certain order and measure light intensity respectively, we can calculate the intensity of transmitted light. According to the intensity results before and after the reaction, we obtain the cell density and fluorescent intensity in the solution.
The 3D-printed cuvette brace offered space for LED and photosensor to be attached. The space for the cuvette is slightly larger than the size of cuvette, leaving extra space for vibration. During the measurement, inlay the cuvette into the fitting hole in order to fix it. The 3D-printed shell is a box with four interfaces attached to the brace. The baffle plate covers and protects details of the system, leaving a hole for the cuvette.
Processor
Beetle-￥49
we chose a small MCU - Beetle based on Arduino Leonardo as processor. It is very small in size, only 20mm by 22mm. In addition, it has a microUSB which can be burned directly, and it can also be connected by wire directly, which is very convenient for debugging.
Photosensitive Sensor
TSL235R-￥10*2
The same as Team Aachen, we choose this photosensitive sensor for its small size, which is only 4.6*2.6*4.6
LED
We use 605nm and 485nm LED, which variance is less than 5nm.
Temperature Sensor
LM35DZ is a cheap and fine temperature sensor, we use it to remind the users to put the yeast in a proper temperature for cultivation.
Power Module
For portable purposes, we use both batteries and power cords for power supply. Specifically, we use a 150mAh battery, a power management module suitable for lithium battery, and a micro USB interface for charging. It is connected to a button, which is clicked for start and double - clicked for shutdown.
Display Screen
In order to display the results visually, we choose to use the display screen to demonstrate the results. We choose a small display screen,0.96 inch, which uses the IIC communication protocol
Total
| Component | Quantity | Total Price |
|---|---|---|
| Beetle | 1 | ￥49 |
| TSL235 | 2 | ￥20 |
| LED | 605nm,485nm | ￥10 |
| Light filter | Scarlet*2,Light Green | ￥0.75 |
| LM35 | 1 | ￥4.7 |
| Battery | 1 | ￥9.9 |
| Power Module | 1 | ￥12.8 |
| MicroUSB | 1 | ￥49 |
| OLED Displayer | 1 | ￥12.7 |
| 3D printed parts (material) | 4 | ￥5 |
| self-locking switch key | 1 | ￥0.26 |
| Wires(AWG30) | 3 | ￥3 |
| Cuvette 4.5mL | 1 | ￥0.7 |
| button cell | 1 | ￥8.8 |
| button cell holder | 1 | ￥0.91 |
| vibration motor | 1 | ￥1.78 |
| Total | 135.7($20.67) |
manufacture
cuvettes
- use a four-sided smooth cuvette for laser cutting to make it 30mm high.
- Buy or make a proper cover for it.
- Prepare some PTFE membrane
Vibration device
- Vibration motor
- Button battery
- Button battery holder
Connect the holder and motor, and fix it with adhesive. Put in the battery when use it.
Testing device
- 3D printing file for the shell and stent
- 3D Pringting Files
- You can change shape of holes in the model to fit your buttons and microUSB
- Try to use a lightproof material for 3D printing. If you don’t have one, just try to make it light-tight.
- Wiring
- Analog
- LED*2(remember to have a 10K resistance in series!) - A9 A10
- LM35 – A11
- Digital
- Button “Go” – D9
- TSL235 – interrupt pin D0, D1(same to Serial1 Tx, Rx)
- IIC
- SCK,SDA to displayer (remove pin header to save the room)
- Power Module
- Programme
Connect the holder and motor, and fix it with adhesive. Put in the battery when use it.
Future Plan
- Use more proper filters to improve the precision
- Try to find a better solution for vibration
- Perfect the appearance
