Difference between revisions of "Team:Peking/Parts"

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                             <h3 class="classic-title"><a href="http://parts.igem.org/Part:BBa_K2601010">BBa_K2601010</a></h3>
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<h3 class="title><p>Schematic diagram</p></h3>
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Revision as of 17:36, 16 October 2018

Parts

Favorite Parts

BBa_K2601010

Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein

BBa_K2601010

Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein

Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.


BBa_K2601011

Fig. 2. 3A: Triple SpyTag SUP: Super Uranyl-binding Protein

Super Uranyl-binding Protein (SUP) was fused to 4*(VPGVG) spaced triple SpyTag. The fused protein is used to generate the uranyl-binding capacity in our polymer network system. Paired with triple SpyCatcher (BBa_K1989001) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.


BBa_K2601012

Fig. 3. 3A: Triple SpyTag mSA: Monomeric streptavidin

Triple SpyCatcher was spaced by 15*(VPGVG). Paired with triple SpyTag with SUP (BBa_K1989000) and triple SpyTag with mSA (BBa_K1989002), our polymer network system is constructed.


Part Table
BBa_ Part
K2601000 Promoter-tet07
K2601001 Promoter-PDH3
K2601002 SUMO
K2601003 SIM
K2601004 HOTag3
K2601005 HOTag6
K2601007 Frb-yEGFP
K2601008 FKBP-yEGFP
K2601010 Frb-yEGFP-HOTag6
K2601011 FKBP-yEGFP-HOTag3
K2601012 SUMO-yEGFP-HOtag3
K2601021 Tet07-Frb-yEGFP
K2601023 PDH3-Frb-yEGFP
K2601025 Tet07-FKBP-yEGFP
K2601026 TEF1-FKBP-yEGFP
K2601027 PDH3-FKBP-yEGFP
K2601032 Tet07-Frb-yEGFP-HOTag6
K2601033 TEF1-Frb-yEGFP-HOTag6
K2601034 PDH3-Frb-yEGFP-HOTag6
K2601037 TEF1-FKBP-yEGFP-HOTag3
K2601038 PDH3-FKBP-yEGFP-HOTag3
K2601040 Tet07-SUMO-yEGFP-HOtag3
K2601041 TEF1-SUMO-yEGFP-HOtag3
K2601042 PDH3-SUMO-yEGFP-HOtag3
K2601054 PDH3-Frb-crtE-HOTag6
K2601056 Tet07-Frb-crtYB-HOTag6
K2601058 PDH3-Frb-crtYB-HOTag6
K2601060 SIM-crtE-Hotag6
K2601061 SIM-crtYB-Hotag6
Part Collection

2018 Peking iGEM team members devoted themselves to constructing a part collection that can drive phase separation. We have not only submitted all of new basic HOTag parts, but also provided multiple composite parts combining HOTags with other protein-protein interaction modules and fluorescent reporters. The basic part, HOTag3, is a homo-oligomeric short peptide containing only 30 amino acids. It has high stoichiometry, forming hexamer spontaneously. The HOTag3, together with another tetrameric HOTag6, can robustly drive phase separation upon protein-protein interaction. Protein-protein interaction is achieved by our dimerization parts, including FKBP/Frb and SUMO/SIM. Functions of all the parts were thoroughly tested. Some thermodynamic and kinetic properties of the parts were characterized as well. We believe the HOTag is useful tool for other iGEM teams to investigate protein phase separation and design synthetic organelles.