Difference between revisions of "Team:TJU China"

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     <div class="head">Dynamic Model of Heavy Metal Detection Biosensor</div>
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    <div class="subhead">Minghui Yin,Sherry Dongqi Bao<br>TianJin University<br>October 15,2018</div>
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    <div class="title">1  Introduction</div>
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    <div class="word">Modeling is a powerful tool in synthetic biology. It provides us with a necessary engineering approach to characterize our pathways
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         quantitatively and predict their performance,thus help us test and modify our design.Through the dynamic model of heavy-metal detection biosensor,we hope to gain insights into the characteristics of our whole circuit's dynamics.
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     <div class="title">2  Methods</div>
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     <div class="subtitle">2.1 Analysis of metabolic pathways</div>
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    <div class="figure">Figure 1: Metabolic pathways related to plasmid#1</div>
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    <div class="word">At the beginning, on the plasmid#1, the promoter $P_{arsR}$ isn't bound with ArsR,thus it is active.ArsR and smURFP are transcribed and translated under the control of the  
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        promoters $P_{arsR_{u}}$ and $P_{arsR_{d}}$,with subscript u and d representing upstream and downstream separately.The subscript l of smURFP in the equation means leaky expression without
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        the expression of $As^{3+}$.As ArsR is expressed gradually,it will bind with the promoter $P_{arsR}$ and make it inactive.[1]</div>
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            &nbsp;&nbsp;&nbsp;&nbsp; This year, the CRISPR-Cas family is the protagonist in our story series. The old member, dCas9,
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            is the enhancer for the heavy-metal detection based on E. coli, while the newbie, Cas12a, is a worker for the
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            high-throughput cancer-related SNP detection chip. We have also built a "highway" for tracking and delivering
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            the Cas9/sgRNA complex in mammalian cells, and we try to apply it to manipulate the mitochondrial genome.
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    <div class="word">On the plasmid#2,the fusion protein of dCas9 and RNAP(RNA polymerase) are produced after transcription and translation,and sgRNA is produced after transcription.<div>
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$P_{arsR_{d}}$
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                Click the medals to see how we met
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$As^{3+}$
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<div class="equation"> \(P_{J23104} \xrightarrow {k_{tx1}}  P_{J23104} + mRNA_{ArsR}\)</div> <div class="number">(1)</div>  
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            <div>天津大学</div>
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            <div>TianJin University</div>
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            <div>天津大学生命科学学院</div>
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            <div>School of life Sciences,</div>
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            <div>TianJin University</div>
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        <div class="home_copyright">@IGEM 2018 TJU_China.All Rights Reserved.丨Contact us:syq47xx@sina.cn丨(Designed by Peicheng Li)</div>
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Revision as of 18:20, 16 October 2018

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Dynamic Model of Heavy Metal Detection Biosensor
Minghui Yin,Sherry Dongqi Bao
TianJin University
October 15,2018
1 Introduction
Modeling is a powerful tool in synthetic biology. It provides us with a necessary engineering approach to characterize our pathways quantitatively and predict their performance,thus help us test and modify our design.Through the dynamic model of heavy-metal detection biosensor,we hope to gain insights into the characteristics of our whole circuit's dynamics.
2 Methods
2.1 Analysis of metabolic pathways
Figure 1: Metabolic pathways related to plasmid#1
At the beginning, on the plasmid#1, the promoter $P_{arsR}$ isn't bound with ArsR,thus it is active.ArsR and smURFP are transcribed and translated under the control of the promoters $P_{arsR_{u}}$ and $P_{arsR_{d}}$,with subscript u and d representing upstream and downstream separately.The subscript l of smURFP in the equation means leaky expression without the expression of $As^{3+}$.As ArsR is expressed gradually,it will bind with the promoter $P_{arsR}$ and make it inactive.[1]
On the plasmid#2,the fusion protein of dCas9 and RNAP(RNA polymerase) are produced after transcription and translation,and sgRNA is produced after transcription.