Difference between revisions of "Team:Toulouse-INSA-UPS/Basic Part"
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<h1 class="heavy">Basic Parts</h1> | <h1 class="heavy">Basic Parts</h1> | ||
| − | <h2 class="heavy">Parts | + | <h2 class="heavy">Parts Deposited On the Registry</h2> |
<h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/> | <h3>Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)</h3><hr/> | ||
<div style="text-align:center"> | <div style="text-align:center"> | ||
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</div> | </div> | ||
<p> | <p> | ||
| − | This part consists on a CBM3a fused with mSA2, an engineered version of | + | This part consists on a CBM3a fused with mSA2, an engineered version of streptavidin and rhizavidin, on his <i>N-</i>terminus endogenous linker and containing a UAG codon on his <i>C-</i>terminus linker (amber stop codon) for the incorporation of the unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction. |
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668010" target="_blank">page</a>. | <br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668010" target="_blank">page</a>. | ||
</p> | </p> | ||
<br/> | <br/> | ||
| − | <h3>Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) | + | <h3>Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) Fused to mRFP1</h3> |
<hr/> | <hr/> | ||
<div style="text-align:center"> | <div style="text-align:center"> | ||
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</div> | </div> | ||
<p> | <p> | ||
| − | This part results | + | This part results in the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construction is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept. |
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668020" target="_blank">page</a>. | <br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668020" target="_blank">page</a>. | ||
</p> | </p> | ||
<br/> | <br/> | ||
| − | <h3>mTagBFP | + | <h3>mTagBFP Avi-tag</h3><hr/> |
<div style="text-align:center"> | <div style="text-align:center"> | ||
<figure class="figure" style="text-align:center;"> | <figure class="figure" style="text-align:center;"> | ||
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</div> | </div> | ||
<p> | <p> | ||
| − | mTag Blue Fluorescent Protein fused with an | + | mTag Blue Fluorescent Protein fused with an Avi-tag for <i>in vivo</i> biotinylation by BirA. |
<br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668070" target="_blank">page</a>. | <br/>For more details about the design, refer to the corresponding registry <a href="http://parts.igem.org/Part:BBa_K2668070" target="_blank">page</a>. | ||
</p> | </p> | ||
Revision as of 20:29, 16 October 2018
Basic Parts
Parts Deposited On the Registry
Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)
This part consists on a CBM3a fused with mSA2, an engineered version of streptavidin and rhizavidin, on his N-terminus endogenous linker and containing a UAG codon on his C-terminus linker (amber stop codon) for the incorporation of the unnatural amino acid. Note that this part encodes the Cerberus platform if AzF is provided and the appropriate tRNA synthetase is expressed. Otherwise, it encodes for the Orthos construction.
For more details about the design, refer to the corresponding registry page.
Sirius: Cellulose-Binding Modules of Type 3a (CBM3a) Fused to mRFP1
This part results in the fusion of the fluorescent protein mRFP1 to the cellulose binding domain CBM3a. The aim of this construction is to prove the binding of CBM3a to cellulose and to quantify it by fluorescence. Furthermore, the mRFP1 is visible to the naked eye and can therefore be used as a visual proof of concept.
For more details about the design, refer to the corresponding registry page.
mTagBFP Avi-tag
mTag Blue Fluorescent Protein fused with an Avi-tag for in vivo biotinylation by BirA.
For more details about the design, refer to the corresponding registry page.
No dogs were harmed over the course of this iGEM project.
The whole Toulouse INSA-UPS team wants to thank our sponsors, especially:
And many more. For futher information about our sponsors, please consult our Sponsors page.
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