Difference between revisions of "Team:TJU China"

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                         <a href="https://2018.igem.org/Team:TJU_China/Human_Practices">Human Practices</a>
 
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     <div class="head">Dynamic Model of Heavy Metal Detection Biosensor</div>
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    <div class="subhead">Minghui Yin,Sherry Dongqi Bao<br>TianJin University<br>October 15,2018</div>
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    <div class="title">1  Introduction</div>
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    <div class="word">Modeling is a powerful tool in synthetic biology. It provides us with a necessary engineering approach to characterize our pathways
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         quantitatively and predict their performance,thus help us test and modify our design.Through the dynamic model of heavy-metal detection biosensor,we hope to gain insights into the characteristics of our whole circuit's dynamics.
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     <div class="title">2  Methods</div>
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     <div class="subtitle">2.1 Analysis of metabolic pathways</div>
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    <div class="figure">Figure 1: Metabolic pathways related to plasmid#1</div>
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    <div class="word">At the beginning, on the plasmid#1, the promoter $P_{arsR}$ isn't bound with ArsR,thus it is active.ArsR and smURFP are transcribed and translated under the control of the  
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        promoters $P_{arsR_{u}}$ and $P_{arsR_{d}}$,with subscript u and d representing upstream and downstream separately.The subscript l of smURFP in the equation means leaky expression without
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        the expression of $As^{3+}$.As ArsR is expressed gradually,it will bind with the promoter $P_{arsR}$ and make it inactive.[1]</div>
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            ABSTRACT
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            &nbsp;&nbsp;&nbsp;&nbsp; This year, the CRISPR-Cas family is the protagonist in our story series. The old member, dCas9,
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            is the enhancer for the heavy-metal detection based on E. coli, while the newbie, Cas12a, is a worker for the
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            high-throughput cancer-related SNP detection chip. We have also built a "highway" for tracking and delivering
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            the Cas9/sgRNA complex in mammalian cells, and we try to apply it to manipulate the mitochondrial genome.
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    <div class="word">On the plasmid#2,the fusion protein of dCas9 and RNAP(RNA polymerase) are produced after transcription and translation,and sgRNA is produced after transcription.<div>
 
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            ACHIEVEMENTS
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$P_{arsR_{d}}$
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                Click the medals to see how we met
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                <br> the iGEM medal requirements for 2018!</div>
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$As^{3+}$
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<div class="equation"> \(P_{J23104} \xrightarrow {k_{tx1}}  P_{J23104} + mRNA_{ArsR}\)</div> <div class="number">(1)</div>  
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            <div>天津大学</div>
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            <div>TianJin University</div>
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            <div>天津大学生命科学学院</div>
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            <div>School of life Sciences,</div>
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            <div>TianJin University</div>
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        <div class="home_copyright">@IGEM 2018 TJU_China.All Rights Reserved.丨Contact us:syq47xx@sina.cn丨(Designed by Peicheng Li)</div>
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Revision as of 00:32, 17 October 2018

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ABSTRACT
     This year, the CRISPR-Cas family is the protagonist in our story series. The old member, dCas9, is the enhancer for the heavy-metal detection based on E. coli, while the newbie, Cas12a, is a worker for the high-throughput cancer-related SNP detection chip. We have also built a "highway" for tracking and delivering the Cas9/sgRNA complex in mammalian cells, and we try to apply it to manipulate the mitochondrial genome.
ACHIEVEMENTS
Click the medals to see how we met
the iGEM medal requirements for 2018!
天津大学
TianJin University
天津大学生命科学学院
School of life Sciences,
TianJin University
@IGEM 2018 TJU_China.All Rights Reserved.丨Contact us:syq47xx@sina.cn丨(Designed by Peicheng Li)