Difference between revisions of "Team:NCKU Tainan/Design"

Line 188: Line 188:
 
                                     <p class="pcontent">We selected two pH sensitive promoter from <i>E. coli</i>:  
 
                                     <p class="pcontent">We selected two pH sensitive promoter from <i>E. coli</i>:  
 
                                         P<sub>asr</sub> and P<sub>gadA</sub>. P<sub>gadA</sub> will be induced under neutral condition while P<sub>asr</sub> will be induced under acidic condition.  
 
                                         P<sub>asr</sub> and P<sub>gadA</sub>. P<sub>gadA</sub> will be induced under neutral condition while P<sub>asr</sub> will be induced under acidic condition.  
                                         We cloned a GFP and sfGFP gene downstream of these promoters respectively, whose product will express green fluorescence once the promoter has been activated. For the design of P<sub>gadA</sub> sensing system, we took the previous constructed P<sub>gadA</sub>biobrick <a href="http://parts.igem.org/Part:BBa_K1962013"
+
                                         We cloned a GFP and sfGFP gene downstream of these promoters respectively, whose product will express green fluorescence once the promoter has been activated. For the design of P<sub>gadA</sub> sensing system, we took the previous constructed P<sub>gadA</sub> biobrick <a href="http://parts.igem.org/Part:BBa_K1962013"
 
                                             style="color:#28ff28;">BBa_K1962013</a> from <a href="https://2016.igem.org/Team:Dundee"
 
                                             style="color:#28ff28;">BBa_K1962013</a> from <a href="https://2016.igem.org/Team:Dundee"
 
                                             style="color:#28ff28;">2016 iGEM Dundee team</a> as our reference. We also improve the P<sub>gadA</sub> biobrick to enhance the expression of GFP.
 
                                             style="color:#28ff28;">2016 iGEM Dundee team</a> as our reference. We also improve the P<sub>gadA</sub> biobrick to enhance the expression of GFP.

Revision as of 08:15, 17 October 2018

Design

Follow us

Contact us

igem.ncku.tainan@gmail.com
No.1, Daxue Rd., East Dist., Tainan City 701, Taiwan (R.O.C.)