Difference between revisions of "Team:NEU China B/Improve"

Line 5: Line 5:
  
 
<H2>
 
<H2>
−
BBa_k2824008:Lldr-T7-lldPRD operon promoter-GFP
+
BBa_k2824008:Lldr-T7-lldPRD operon promoter-GFP
−
</H2>
+
</H2>
−
<p>
+
<div class="p">
−
<br>The role of this part is to try to control the concentration of lactic acid on the LLDPRD promoter under the
+
<br>The role of this part is to try to control the concentration of lactic acid on the LLDPRD promoter under the
−
control of quorum sensingg. In the case of LLdr gene expression, the subsequent gene expression was initiated.
+
control of quorum sensingg. In the case of LLdr gene expression, the subsequent gene expression was initiated.
−
<br>This part also is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
+
<br>This part also is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
−
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. Comparing with the
+
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. Comparing with the
−
T7-lldPRD operon promoter-GFP part, the improvement of this part is that the LLDR is added to part and placed under
+
T7-lldPRD operon promoter-GFP part, the improvement of this part is that the LLDR is added to part and placed under
−
the control of two promoters respectively to form a double expression regulatory system. This comparison with the two
+
the control of two promoters respectively to form a double expression regulatory system. This comparison with the two
−
plasmids working together is more convenient for testing the success of the system.
+
plasmids working together is more convenient for testing the success of the system.
−
<br>We can easily find that the Lldr-T7-lldPRD operon promoter-GFP part are in a higher level of expression comparing
+
<br>We can easily find that the Lldr-T7-lldPRD operon promoter-GFP part are in a higher level of expression comparing
−
to the lldPRD operon promoter-GFP when the lactate concentration is lower than nearly 1.5m mol/L. Considering that
+
to the lldPRD operon promoter-GFP when the lactate concentration is lower than nearly 1.5m mol/L. Considering that
−
the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter
+
the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter
−
can enhance the signal intensity for our experiments.
+
can enhance the signal intensity for our experiments.
−
</p>
+
<div>
−
 
+
−
<h2>
+
<h2>
−
BBa_k2824006:T7-lldPRD operon promoter-GFP
+
BBa_k2824006:T7-lldPRD operon promoter-GFP
−
</h2>
+
</h2>
−
<p>
+
<div class="p">
−
<br>LLDPRD can sense the presence of lactic acid, in the absence of lactic acid, only a lower background level of
+
<br>LLDPRD can sense the presence of lactic acid, in the absence of lactic acid, only a lower background level of
−
expression, but in the presence of lactic acid can open expression, gene transcription. We added the GFP gene into
+
expression, but in the presence of lactic acid can open expression, gene transcription. We added the GFP gene into
−
the gene and used green fluorescence as a marker to recognize the presence of lactic acid. In addition, as a strong
+
the gene and used green fluorescence as a marker to recognize the presence of lactic acid. In addition, as a strong
−
promoter, T7 can improve the expression level of the target gene.
+
promoter, T7 can improve the expression level of the target gene.
−
<img src="https://static.igem.org/mediawiki/2018/e/ea/T--NEU_China_B--ct8.png">
+
<img src="https://static.igem.org/mediawiki/2018/e/ea/T--NEU_China_B--ct8.png">
−
<img src="https://static.igem.org/mediawiki/2018/3/34/T--NEU_China_B--improve2.png">
+
<img src="https://static.igem.org/mediawiki/2018/3/34/T--NEU_China_B--improve2.png">
−
<br>This part is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
+
<br>This part is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a
−
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. We added a sequence
+
lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. We added a sequence
−
of T 7 promoter and GFP fluorescent protein gene before and after this part Separately. It can effectively improve
+
of T 7 promoter and GFP fluorescent protein gene before and after this part Separately. It can effectively improve
−
the expression of the target gene and detect whether the target gene is expressed or not. At the same time, it can
+
the expression of the target gene and detect whether the target gene is expressed or not. At the same time, it can
−
accurately determine the amount of the target gene. This allows the overall work of our system to be reflected in
+
accurately determine the amount of the target gene. This allows the overall work of our system to be reflected in
−
digital form.
+
digital form.
−
<br>In the following figure, we compared the expression of lldPRD operon promoter-GFP and T7-lldPRD operon
+
<br>In the following figure, we compared the expression of lldPRD operon promoter-GFP and T7-lldPRD operon
−
promoter-GFP. The T7 promoter could enhance gene expression when the concentration of lactic acid was less than 1 m
+
promoter-GFP. The T7 promoter could enhance gene expression when the concentration of lactic acid was less than 1 m
−
mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can
+
mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can
−
given that the T7 promoter can enhance the signal intensity for our experiments
+
given that the T7 promoter can enhance the signal intensity for our experiments
−
<img src="https://static.igem.org/mediawiki/2018/3/33/T--NEU_China_B--improve3.png">
+
<img src="https://static.igem.org/mediawiki/2018/3/33/T--NEU_China_B--improve3.png">
−
<img src="https://static.igem.org/mediawiki/2018/c/c6/T--NEU_China_B--improve4.png">
+
<img src="https://static.igem.org/mediawiki/2018/c/c6/T--NEU_China_B--improve4.png">
−
<img src="https://static.igem.org/mediawiki/2018/8/8d/T--NEU_China_B--improve5.png">
+
<img src="https://static.igem.org/mediawiki/2018/8/8d/T--NEU_China_B--improve5.png">
−
</p>
+
<div>
−
</html>
+
</html>

Revision as of 14:30, 17 October 2018

Ruby - Responsive Corporate Tempalte

BBa_k2824008:Lldr-T7-lldPRD operon promoter-GFP


The role of this part is to try to control the concentration of lactic acid on the LLDPRD promoter under the control of quorum sensingg. In the case of LLdr gene expression, the subsequent gene expression was initiated.
This part also is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. Comparing with the T7-lldPRD operon promoter-GFP part, the improvement of this part is that the LLDR is added to part and placed under the control of two promoters respectively to form a double expression regulatory system. This comparison with the two plasmids working together is more convenient for testing the success of the system.
We can easily find that the Lldr-T7-lldPRD operon promoter-GFP part are in a higher level of expression comparing to the lldPRD operon promoter-GFP when the lactate concentration is lower than nearly 1.5m mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter can enhance the signal intensity for our experiments.

BBa_k2824006:T7-lldPRD operon promoter-GFP


LLDPRD can sense the presence of lactic acid, in the absence of lactic acid, only a lower background level of expression, but in the presence of lactic acid can open expression, gene transcription. We added the GFP gene into the gene and used green fluorescence as a marker to recognize the presence of lactic acid. In addition, as a strong promoter, T7 can improve the expression level of the target gene.
This part is constructed based on the part lldRO1-plldR-lldRO2 (BBa_k82000) . lldRO1-plldR-lldRO2 part is a lactic acid regulated promoter that is open to gene transcription in the presence of lactic acid. We added a sequence of T 7 promoter and GFP fluorescent protein gene before and after this part Separately. It can effectively improve the expression of the target gene and detect whether the target gene is expressed or not. At the same time, it can accurately determine the amount of the target gene. This allows the overall work of our system to be reflected in digital form.
In the following figure, we compared the expression of lldPRD operon promoter-GFP and T7-lldPRD operon promoter-GFP. The T7 promoter could enhance gene expression when the concentration of lactic acid was less than 1 m mol/L. Considering that the lactate concentration in yogurt is generally no more than 1m mol/L, the conclusion can given that the T7 promoter can enhance the signal intensity for our experiments