Difference between revisions of "Team:SJTU-BioX-Shanghai/Interview"

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                  Integrated Part
 
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             <p><strong>Instructions: you have the change not only the main text, but also have to modify the text in the navgation bar on the left side. Now put your content here and do the same for the following sections. </strong>xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx  xxx xxx xxx xxx xxx Note template is here ---  
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<p>Key Words: Care<br>
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                <span class="footnote_link">OD
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Methods: Literature + Questionnaire + Interview<br>
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Places: Shanghai Sixth People's Hospital + Ottawa Civic Hospital/General Hospital<br>
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                        <span class="footnote_header">OD</span>
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</p>
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                        <span class="footnote_txt">Optical density</span>
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<h3>
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            .</p>
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                  Mission One——Interview in Shanghai
 +
                </a>
 +
            </h3>
 +
             <p>Time: Afternoon, August 13th, 2018<br>
 +
Place: GI endoscopy division of Shanghai Sixth People’s Hospital<br>
 +
Interviewee: Doctor Zheng Haiming<br>
 +
Interviewer: iGEM2018 SJTU-BioX-Shanghai team mumber Qingwei Fang, Shiyu Sun <br>
 +
Topic: Clinical application of colonoscopy<br>
 +
The interviews<br>
 +
1.What are the symptoms patients have that come over to do colonoscopy in general?<br>
 +
①Changes in the character of excrement (including shape, number, bleeding, etc.);<br>
 +
②Physical examination (thickening of the intestinal wall, mass);<br>
 +
③Most from gastroenterology dept or general surgery department<br>
 +
2.What is general examination result?<br>
 +
①No obvious abnormality<br>
 +
②In pathological conditions, intestinal polyp is the most, followed by inflammation, then tumor.<br>
 +
③In the case of cancer, most patients are found to be in an advanced stage.<br>
 +
④Early tumors account for 60% of the tumors (early + late)  found in Japan but less than 10% in China.<br>
 +
3.What is the effect of colonoscopy for early detection of colon cancer?<br>
 +
①Almost is the only way to detect early colon tumors<br>
 +
②The early marker of colon tumors -polyps can be found. Polyps are responsible for 80% of colorectal cancer (non-hereditary).<br>
 +
③In the colonoscopy, the biopsy forceps attached to the colonoscopy can be used to remove the polyps and prevent further development.<br>
 +
4. Is the colonoscopy included in the general medical examination? <br>
 +
Not included.<br>
 +
5. Is the colonoscopy included in the optional inspection program, and if so, how many people will selecte?<br>
 +
Yes., but you need to make an appointment in advance (anesthesia colonoscopy: 2-3 months, common colonoscopy, 1-2 months). 1-2%.<br>
 +
6. What are the reasons for hindering the colonoscopy into a widespread one?<br>
 +
①The popularization of medical knowledge is insufficient, and many people do not know about items with colonoscopy;<br>
 +
②Afraid of colonoscopy;<br>
 +
③Medical resources are limited. One time of colonoscopy is about 20-30 minutes, and a skilled colonoscopy doctor can only examine about 20 patients a day. The demand for endoscopy doctors in China is about 200,000-300,000, but currently there are only about 2-3 million.<br>
 +
7. What is the impact and degree of pain of colonoscopy and other intestinal examinations?<br>
 +
①Colonoscopy: entering from the anus;<br>
 +
②Enteroscopy: entering from the mouth and anus, docking; a enteroscopy should take 2-3 hours; charge 2-3k RMB; painful; hospitals are less profitable and most are reluctant to do it;<br>
 +
③Capsule endoscopy: can not take biopsy; uncontrolled; 3k RMB; not suitable for large intestine examination (can not see, power is not enough).<br>
 +
8. What are other clinical examination methods of colonoscopy other than colonoscopy?<br>
 +
①There is currently no way to completely replace colonoscopy;<br>
 +
②Abdominal enhancement CT;<br>
 +
③Barium enema (can not take biopsy, not as clear as the colonoscopy).<br>
 +
9. What is the ratio of anesthesia colonoscopy to normal colonoscopy?<br>
 +
  1:1<br>
 +
10. What assessments are needed before an anesthesia colonoscopy? What kind of people is not suitable for anesthesia colonoscopy?<br>
 +
①Body type: can not be too fat (anesthesia for too fat people may have breathing risk); <br>
 +
②ECG: can not have inflammatory arrhythmia, ventricular arrhythmia, and have done a heart stent.<br>
 +
③Medical history: no allergies, asthma, high blood pressure, diabetes (the heart is generally damaged, and need to control the diet 3 days before the colonoscopy, can not resist);<br>
 +
④Prolonged bleeding time: the risk of major bleeding is not suitable for colonoscopy.<br>
 +
11. What is the side effects of anesthesia?<br>
 +
①Respiratory depression; <br>
 +
②Blood oxygen reduction; <br>
 +
③Heart rate reduction;<br>
 +
④Major side effects: cough, heart rate disorders, etc.<br>
 +
12. What is the side effect after the colonoscopy?<br>
 +
①General: abdominal distension (aerate the patient's intestines when inspect);<br>
 +
②Serious: collapse (a, stimulating vagal reflexes, nausea; b, colonoscopy 3d control diet; hypoglycemia; c, pain).<br>
 +
13. What are the requirements for the patient's diet before the colonoscopy?<br>
 +
①Slag and semi-flow diet two days before check and treat, do not drink milk and soy milk;<br>
 +
②Liquid diet the day before check;<br>
 +
③Fasting in the check day morning ;<br>
 +
14. What are the key people who doctor want them take a colonoscopy?<br>
 +
①People over 40 years old;<br>
 +
②Tool contains blood;<br>
 +
③Family history of tumors (all tumor types, causes: lung cancer, stomach cancer, intestinal cancer are three high-grade tumors);<br>
 +
④Had intestinal polyps before;<br>
 +
⑤Appendectomy patients (appendectomy is easy to cause intestinal flora disorder, increase the risk of intestinal cancer).<br>
 +
15. As a clinician, are there any hopes and suggestions for improving the inspection method?<br>
 +
①In the colonoscopy, the operation of the rectal intestine requires the operator to learn for a long time to master, which is difficult;<br>
 +
②Colonoscopy operation requires operator to stand for a long time, thus the physical requirements for the doctor is higher. In addition, due to the long-term holding of the colonoscopy, the colonoscopy doctor has occupational diseases in joint and finger injuries. I hope there can be some methods can reduce the physical burden of the doctor.<br>
 +
③Doctors need to be highly concentrated when do colonoscopy, and can not miss the diseased tissue. I hope that the instrument can be used to help doctors find out the lesions to reduce the burden on doctors.<br>
 +
16. From the doctor's point of view, what kind of examination the patient is more willing to accept, not too excluded? <br>
 +
Safe and painless.<br>
 +
</p>
 +
  <h3>
 +
                <a id="section1">
 +
                    <span class="place_holder"></span>
 +
                  Mission Two——Interview in Ottawa
 +
                </a>
 +
            </h3>
 +
<p>
 +
Time: Afternoon, August 16th, 2018<br>
 +
Place: Ottawa Civic Hospital, Cancer Centre<br>
 +
Ottawa General Hospital, Oncology department<br>
 +
Interviewee: Dr.Davis and the sick population of the day<br>
 +
Interviewer: iGEM2018 SJTU-BioX-Shanghai team mumber Bozitao Zhong, Shiyu Bai<br>
 +
Topic: The current dilemma of clinical diagnosis of colon cancer<br>
 +
The interviews<br>
 +
<br>
 +
<strong>1. Interview with Professor of Ottawa Hospital<br></strong>
 +
According to the professor, we learned about the various methods of early diagnosis of colon cancer, and the problems faced by these means:<br>
 +
<br></p>
 +
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                            <p>Colonoscope</p>
 +
                            <img />
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                            <p>Advantages: Colonoscopy is more accurate than barium enema X-ray, especially when detecting small polyps. If colon polyps are found, they are usually removed by colonoscopy and sent for pathological analysis. The pathologist examines the polyps under the microscope to check for cancer.<br>
 +
Disadvantages: Most of the polyps that are removed by colonoscopy are benign, and many are precancerous lesions. Removal of precancerous polyps prevents colon cancer from developing of these polyps in the future. But this is not an efficient method!</p>
 +
                         
 +
 +
                        </div>
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                            <p>Sigmoidoscopy</p>
 +
                            <img />
 +
                       
 +
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 +
                            <p>Sigmoidoscopy is the process of examining the left colon and rectum using a shorter range of dimensions.<br>
 +
Advantages: It is easier to prepare and perform than a full colonoscopy, and sigmoidoscopy can be used for polypectomy and cancer biopsy.<br>
 +
Disadvantages: However, it has significant limitations in not being able to assess right and lateral colons.<br></p>
 +
                        </div>
 +
                    </li>
 +
                    <li class="storage_box_ele">
 +
                        <div class="storage_header">
 +
                       
 +
                            <p>CEA carcinoembryonic antigen method</p>
 +
                            <img />
 +
                       
 +
                        </div>
 +
                       
 +
                        <div class="storage_text">
 +
                           
 +
                            <p>Advantages: Indirect. If cancer is suspected, medical personnel can obtain the Carcinoembryonic Antigen (CEA) - called the "tumor marker" to do blood test. CEA is a substance produced by some colon and rectal cancer cells as well as some other types of cancer. Colon cancer patients have higher levels of CEAS.<br>
 +
Disadvantages: Not all patients’ CEA is elevated though their cancer has spread. (Some colon cancers do not produce CEA.) In addition, some patients without cancer may also have high levels of CEA.<br></p>
 +
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 +
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 +
                   
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 +
 +
 +
<p>It can be roughly presented in the following table:<br></p>
 +
<p>
 +
<br>
 +
Later, we learned from the professors about the current incidence of colon cancer in North America: According to the World Health Organization, colon cancer is already the second most common cancer in both men and women after lung cancer. In North America, the incidence of colon cancer is 2% in people over 50 years of age, and the incidence of colon cancer has little to do with gender. It has been learned from the database of professors in recent years that the age of the onset of colon cancer is decreasing year by year.<br>
 +
All kinds of data suggest that colon cancer needs to receive more attention.<br>
 +
At the same time, after understanding the design of our project, Professor Dr. Davis said that our project is expected to be a convenient and early screening method for colon cancer. According to the World Health Organization (WHO), colorectal cancer is the second most common tumor in men and women after lung cancer. About 2% of people over the age of 50 will eventually develop colorectal cancer in North America. Colorectal cancer often has the same effect on men and women. However, men tend to develop it at a younger age.<br>
 +
<br>
 +
2. Hospital population interview<br>
 +
Our project is for the public, not for the doctor. Therefore, it is necessary for us to interview the masses and listen to the voices of the masses; instead of just referring to the professor's recommendations and the doctor's clinical data.<br>
 +
We divided the mass interview into two parts, interviewing the masses and interviewing patients. The reason why we want to treat the respondents in two categories is because the problems we prepare are different from the information we need.<br>
 +
<br>
 +
The masses are almost undiseased population, which is a direct target for our early diagnosis of colon cancer. We need to introduce to the masses to promote our products and inspection process, and to investigate the acceptance of the masses.<br>
 +
For people<br>
 +
·If this test is included in a routine test, will you participate in the test?<br>
 +
·How much do you think this test should cost?<br>
 +
·What are your expectations for this project?<br>
 +
<br>
 +
For patients, we focus on pain points in patients. Under the condition of not touching the patient's privacy, we try to interviewee the patient's about their living status and the early examination of colon cancer experienced as much as possible from their mouth.<br>
 +
For patients (general level)<br>
 +
·How did you find yourself sick?<br>
 +
·How long have you been sick?<br>
 +
·How long have you been treated?<br>
 +
·How is your treatment effect?<br>
 +
·How do you do with your life after long-time treatment?<br>
 +
·Is there any change that the cancer gives to you?<br>
 +
·From your level of awareness, do you think our project makes sense?<br>
 +
The conclusion is:<br>
 +
With the improvement of science and technology, colon cancer is no longer as painful as before, and most people are acceptable. However, considering the individual tolerance, patients can choose painless colonoscopy according to their own wishes. Painless colonoscopy is performed under general anesthesia. However, it is also necessary to consider the risks of general anesthesia, such as anesthesia accidents, sometimes anesthesia recovery process is longer, and the cost is more expensive.<br>
 +
Before taking the colonoscopy, you need to take laxatives and discharge the excretions in the intestines. This is a painful process: the colonoscopy is still very painful. The tube is pushed from the anus and then the intestines. Keep deepening according to the shape of colon. Because the intestines have ascending colon, descending colon, sigmoid colon, etc., when the tube comes to turning, it is very painful and generally difficult to bear.<br>
 +
One patient revealed that he had had a colonoscopy. After doing it, he did not even have the strength to walk. Patient can choose painless colonoscopy, which is the state of anesthesia, and it can alleviate the pain. The instrument for colonoscopy is a flexible fiber hose with a length of about 140CM. The front end is a fiber hose with a miniature electronic camera with a light source. The rear side is connected with an imaging display. The doctor operates the moving direction of scope according to the image on the display to the intestine. After entering the anus, the colonoscopy passes through the rectum, sigmoid colon, descending colon, transverse colon, ascending colon and cecum.<br>
 +
In order to see the road conditions in the intestine during the examination, the doctor will continue to inflate and pump the intestines in the intestines. The stomach will have a feeling of bloating when inflated. This pain is within an acceptable range. If the above picture can be displayed, you can see that the colonoscopy will go through three curves during the on-going process, the first is the sigmoid colon, the second is the spleen of the descending colon to the transverse colon, and the third is the transverse colon to the hepatic curvature of ascending colon. It is a great test for doctors and patients going through these three corners. If the doctor is skilled and can lightly pass these three corners, the patient will not have much pain. However, if the doctor is not skilled, it will be a painful thing. These three corners, recalled by people who have completed the course, the pain levels from high to low are the spleen, sigmoid colon and hepatic curvature, which means that the second one is the most difficult, which is consistent with the colonoscopy operation skill I found: over the spleen is the most difficult test for doctor's patience and technology. This bend will generally take a long time, and need to try more times technologically. Once the spleen is over, the most difficult part of the colonoscopy is cleared, and the next one should be affordable. When the scope is retreated, it is said that there is no pain.<br>
 +
 +
</p>
 +
<h3>
 +
                <a id="section1">
 +
                    <span class="place_holder"></span>
 +
                  Mission Three——Questionnaire in Shanghai & Ottawa
 +
                </a>
 +
            </h3>
 +
<p>
 +
</p>         
 
             <h2>
 
             <h2>
 
                 <a id="section2">
 
                 <a id="section2">

Revision as of 15:52, 17 October 2018

Interview

Integrated Part

Key Words: Care
Methods: Literature + Questionnaire + Interview
Places: Shanghai Sixth People's Hospital + Ottawa Civic Hospital/General Hospital

Mission One——Interview in Shanghai

Time: Afternoon, August 13th, 2018
Place: GI endoscopy division of Shanghai Sixth People’s Hospital
Interviewee: Doctor Zheng Haiming
Interviewer: iGEM2018 SJTU-BioX-Shanghai team mumber Qingwei Fang, Shiyu Sun
Topic: Clinical application of colonoscopy
The interviews
1.What are the symptoms patients have that come over to do colonoscopy in general?
①Changes in the character of excrement (including shape, number, bleeding, etc.);
②Physical examination (thickening of the intestinal wall, mass);
③Most from gastroenterology dept or general surgery department
2.What is general examination result?
①No obvious abnormality
②In pathological conditions, intestinal polyp is the most, followed by inflammation, then tumor.
③In the case of cancer, most patients are found to be in an advanced stage.
④Early tumors account for 60% of the tumors (early + late) found in Japan but less than 10% in China.
3.What is the effect of colonoscopy for early detection of colon cancer?
①Almost is the only way to detect early colon tumors
②The early marker of colon tumors -polyps can be found. Polyps are responsible for 80% of colorectal cancer (non-hereditary).
③In the colonoscopy, the biopsy forceps attached to the colonoscopy can be used to remove the polyps and prevent further development.
4. Is the colonoscopy included in the general medical examination?
Not included.
5. Is the colonoscopy included in the optional inspection program, and if so, how many people will selecte?
Yes., but you need to make an appointment in advance (anesthesia colonoscopy: 2-3 months, common colonoscopy, 1-2 months). 1-2%.
6. What are the reasons for hindering the colonoscopy into a widespread one?
①The popularization of medical knowledge is insufficient, and many people do not know about items with colonoscopy;
②Afraid of colonoscopy;
③Medical resources are limited. One time of colonoscopy is about 20-30 minutes, and a skilled colonoscopy doctor can only examine about 20 patients a day. The demand for endoscopy doctors in China is about 200,000-300,000, but currently there are only about 2-3 million.
7. What is the impact and degree of pain of colonoscopy and other intestinal examinations?
①Colonoscopy: entering from the anus;
②Enteroscopy: entering from the mouth and anus, docking; a enteroscopy should take 2-3 hours; charge 2-3k RMB; painful; hospitals are less profitable and most are reluctant to do it;
③Capsule endoscopy: can not take biopsy; uncontrolled; 3k RMB; not suitable for large intestine examination (can not see, power is not enough).
8. What are other clinical examination methods of colonoscopy other than colonoscopy?
①There is currently no way to completely replace colonoscopy;
②Abdominal enhancement CT;
③Barium enema (can not take biopsy, not as clear as the colonoscopy).
9. What is the ratio of anesthesia colonoscopy to normal colonoscopy?
1:1
10. What assessments are needed before an anesthesia colonoscopy? What kind of people is not suitable for anesthesia colonoscopy?
①Body type: can not be too fat (anesthesia for too fat people may have breathing risk);
②ECG: can not have inflammatory arrhythmia, ventricular arrhythmia, and have done a heart stent.
③Medical history: no allergies, asthma, high blood pressure, diabetes (the heart is generally damaged, and need to control the diet 3 days before the colonoscopy, can not resist);
④Prolonged bleeding time: the risk of major bleeding is not suitable for colonoscopy.
11. What is the side effects of anesthesia?
①Respiratory depression;
②Blood oxygen reduction;
③Heart rate reduction;
④Major side effects: cough, heart rate disorders, etc.
12. What is the side effect after the colonoscopy?
①General: abdominal distension (aerate the patient's intestines when inspect);
②Serious: collapse (a, stimulating vagal reflexes, nausea; b, colonoscopy 3d control diet; hypoglycemia; c, pain).
13. What are the requirements for the patient's diet before the colonoscopy?
①Slag and semi-flow diet two days before check and treat, do not drink milk and soy milk;
②Liquid diet the day before check;
③Fasting in the check day morning ;
14. What are the key people who doctor want them take a colonoscopy?
①People over 40 years old;
②Tool contains blood;
③Family history of tumors (all tumor types, causes: lung cancer, stomach cancer, intestinal cancer are three high-grade tumors);
④Had intestinal polyps before;
⑤Appendectomy patients (appendectomy is easy to cause intestinal flora disorder, increase the risk of intestinal cancer).
15. As a clinician, are there any hopes and suggestions for improving the inspection method?
①In the colonoscopy, the operation of the rectal intestine requires the operator to learn for a long time to master, which is difficult;
②Colonoscopy operation requires operator to stand for a long time, thus the physical requirements for the doctor is higher. In addition, due to the long-term holding of the colonoscopy, the colonoscopy doctor has occupational diseases in joint and finger injuries. I hope there can be some methods can reduce the physical burden of the doctor.
③Doctors need to be highly concentrated when do colonoscopy, and can not miss the diseased tissue. I hope that the instrument can be used to help doctors find out the lesions to reduce the burden on doctors.
16. From the doctor's point of view, what kind of examination the patient is more willing to accept, not too excluded?
Safe and painless.

Mission Two——Interview in Ottawa

Time: Afternoon, August 16th, 2018
Place: Ottawa Civic Hospital, Cancer Centre
Ottawa General Hospital, Oncology department
Interviewee: Dr.Davis and the sick population of the day
Interviewer: iGEM2018 SJTU-BioX-Shanghai team mumber Bozitao Zhong, Shiyu Bai
Topic: The current dilemma of clinical diagnosis of colon cancer
The interviews

1. Interview with Professor of Ottawa Hospital
 According to the professor, we learned about the various methods of early diagnosis of colon cancer, and the problems faced by these means:

  • Colonoscope

    Advantages: Colonoscopy is more accurate than barium enema X-ray, especially when detecting small polyps. If colon polyps are found, they are usually removed by colonoscopy and sent for pathological analysis. The pathologist examines the polyps under the microscope to check for cancer.
    Disadvantages: Most of the polyps that are removed by colonoscopy are benign, and many are precancerous lesions. Removal of precancerous polyps prevents colon cancer from developing of these polyps in the future. But this is not an efficient method!

  • Sigmoidoscopy

    Sigmoidoscopy is the process of examining the left colon and rectum using a shorter range of dimensions.
    Advantages: It is easier to prepare and perform than a full colonoscopy, and sigmoidoscopy can be used for polypectomy and cancer biopsy.
    Disadvantages: However, it has significant limitations in not being able to assess right and lateral colons.

  • CEA carcinoembryonic antigen method

    Advantages: Indirect. If cancer is suspected, medical personnel can obtain the Carcinoembryonic Antigen (CEA) - called the "tumor marker" to do blood test. CEA is a substance produced by some colon and rectal cancer cells as well as some other types of cancer. Colon cancer patients have higher levels of CEAS.
    Disadvantages: Not all patients’ CEA is elevated though their cancer has spread. (Some colon cancers do not produce CEA.) In addition, some patients without cancer may also have high levels of CEA.

It can be roughly presented in the following table:


Later, we learned from the professors about the current incidence of colon cancer in North America: According to the World Health Organization, colon cancer is already the second most common cancer in both men and women after lung cancer. In North America, the incidence of colon cancer is 2% in people over 50 years of age, and the incidence of colon cancer has little to do with gender. It has been learned from the database of professors in recent years that the age of the onset of colon cancer is decreasing year by year.
All kinds of data suggest that colon cancer needs to receive more attention.
At the same time, after understanding the design of our project, Professor Dr. Davis said that our project is expected to be a convenient and early screening method for colon cancer. According to the World Health Organization (WHO), colorectal cancer is the second most common tumor in men and women after lung cancer. About 2% of people over the age of 50 will eventually develop colorectal cancer in North America. Colorectal cancer often has the same effect on men and women. However, men tend to develop it at a younger age.

2. Hospital population interview
Our project is for the public, not for the doctor. Therefore, it is necessary for us to interview the masses and listen to the voices of the masses; instead of just referring to the professor's recommendations and the doctor's clinical data.
We divided the mass interview into two parts, interviewing the masses and interviewing patients. The reason why we want to treat the respondents in two categories is because the problems we prepare are different from the information we need.

The masses are almost undiseased population, which is a direct target for our early diagnosis of colon cancer. We need to introduce to the masses to promote our products and inspection process, and to investigate the acceptance of the masses.
For people
·If this test is included in a routine test, will you participate in the test?
·How much do you think this test should cost?
·What are your expectations for this project?

For patients, we focus on pain points in patients. Under the condition of not touching the patient's privacy, we try to interviewee the patient's about their living status and the early examination of colon cancer experienced as much as possible from their mouth.
For patients (general level)
·How did you find yourself sick?
·How long have you been sick?
·How long have you been treated?
·How is your treatment effect?
·How do you do with your life after long-time treatment?
·Is there any change that the cancer gives to you?
·From your level of awareness, do you think our project makes sense?
The conclusion is:
With the improvement of science and technology, colon cancer is no longer as painful as before, and most people are acceptable. However, considering the individual tolerance, patients can choose painless colonoscopy according to their own wishes. Painless colonoscopy is performed under general anesthesia. However, it is also necessary to consider the risks of general anesthesia, such as anesthesia accidents, sometimes anesthesia recovery process is longer, and the cost is more expensive.
Before taking the colonoscopy, you need to take laxatives and discharge the excretions in the intestines. This is a painful process: the colonoscopy is still very painful. The tube is pushed from the anus and then the intestines. Keep deepening according to the shape of colon. Because the intestines have ascending colon, descending colon, sigmoid colon, etc., when the tube comes to turning, it is very painful and generally difficult to bear.
One patient revealed that he had had a colonoscopy. After doing it, he did not even have the strength to walk. Patient can choose painless colonoscopy, which is the state of anesthesia, and it can alleviate the pain. The instrument for colonoscopy is a flexible fiber hose with a length of about 140CM. The front end is a fiber hose with a miniature electronic camera with a light source. The rear side is connected with an imaging display. The doctor operates the moving direction of scope according to the image on the display to the intestine. After entering the anus, the colonoscopy passes through the rectum, sigmoid colon, descending colon, transverse colon, ascending colon and cecum.
In order to see the road conditions in the intestine during the examination, the doctor will continue to inflate and pump the intestines in the intestines. The stomach will have a feeling of bloating when inflated. This pain is within an acceptable range. If the above picture can be displayed, you can see that the colonoscopy will go through three curves during the on-going process, the first is the sigmoid colon, the second is the spleen of the descending colon to the transverse colon, and the third is the transverse colon to the hepatic curvature of ascending colon. It is a great test for doctors and patients going through these three corners. If the doctor is skilled and can lightly pass these three corners, the patient will not have much pain. However, if the doctor is not skilled, it will be a painful thing. These three corners, recalled by people who have completed the course, the pain levels from high to low are the spleen, sigmoid colon and hepatic curvature, which means that the second one is the most difficult, which is consistent with the colonoscopy operation skill I found: over the spleen is the most difficult test for doctor's patience and technology. This bend will generally take a long time, and need to try more times technologically. Once the spleen is over, the most difficult part of the colonoscopy is cleared, and the next one should be affordable. When the scope is retreated, it is said that there is no pain.

Mission Three——Questionnaire in Shanghai & Ottawa

Section2

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Section3

xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx xxx. The text-link template is here.

2018 Interlab Plate Reader Protocol
Protocols/Transformation

section4

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Fig 1. The particle standard curve obtained form the 2nd calibration experiment.

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The table template is here.

Table 1. Colony forming units per 0.1 OD600

samples dilution factor CFU/mL
8×104 8×105 8×106
1.1 TNTC 48 11 3.84E+07
1.2 248 41 10 3.28E+07
1.3 172 54 5 4.32E+07
2.1 TNTC 143 20 1.14E+08
2.2 TNTC 153 25 1.22E+08
2.3 TNTC 151 18 1.21E+08
3.1 TNTC 119 16 9.52E+07
3.2 TNTC 125 19 1.00E+08
3.3 TNTC 89 18 7.12E+07
4.1 TNTC 209 16 1.67E+08
4.2 TNTC 130 17 1.04E+08
4.3 TNTC 164 10 1.31E+08

Section5

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