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Revision as of 00:03, 18 October 2018

Alternative Roots/Protocols

Alternative Roots

Parts Overview

Overview

Parts Used

Here we list the parts used in our study.



New Parts

Name Type Description Designer Length(bp)
BBa_I20270 Coding sequence Positive control for the interLab study Jason Kelly 919
BBa_J364000 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364001 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364002 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364007 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364008 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_J364009 Coding sequence GFP gene for the interlab study Traci Haddock-Angelli 918
BBa_R0040 Regulatory sequence TetR repressible promoter for the interlab study June Rhee, Connie Tao, Ty Thomson, Louis Waldman 54
BBa_J04450 Coding sequence RFP gene for the interlab study Tamar Odle 1069
BBa_K1033001 Coding sequence Catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A Karl Holdar 1708
BBa_K1497000 Coding sequence Catalyses the reaction from naringenin chalcone to naringenin. Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen 726
BBa_K1497001 Coding sequence Catalyses the reaction from 4-Coumaroyl-CoA to naringenin chalcone. Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen 1197

Existing Parts

BBa_K2797002 Composite Composite part for streptomycin resistance Frank Eardley 908
BBa_K2797014 Coding sequence Catalyses the formation of p-coumaric acid from tyrosine Heather Bottomley 1596
BBa_K2797003 Basic mNeonGreen fluorescent protein gene codon optimised for expression in E. coli Kyle Stanforth 711
BBa_K2797004 Composite Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797005 Composite Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797006 Composite Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797007 Composite Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 909
BBa_K2797008 Composite Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter Kyle Stanforth 910
BBa_K2797013 Plasmid vector High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard Kyle Stanforth 3073




References & Attributions

Attributions: Frank Eardley, Heather Bottomley and Kyle Stanforth