Peking 2018 joined the fifth Interlab measurement study. This year we helped to answer this question: Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD?
The introduction of this year Interlab: https://2018.igem.org/Measurement/InterLab
Plate Reader: Perkin Elemer EnSpire TM Multilabel Reader 2300
Flow Cytometry: BD LSRFortessa TM Cell Analyzer
96 - Well Pate: Corning Incorporated Costar®️ 3603
![](https://static.igem.org/mediawiki/2018/5/5a/T--Peking--ludox.png)
![](https://static.igem.org/mediawiki/2018/3/3a/T--Peking--standcurve1.png)
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(a) Particle Standard Curve - Linear | (b) Particle Standard Curve - Log Scale |
Figure. 3 The result of particle standard curve
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![](https://static.igem.org/mediawiki/2018/2/25/T--Peking--fluroscencestandcurve1.png)
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(a) Fluorescence Standard Curve - Linear | (b) Fluorescence Standard Curve - Log Scale |
Figure. 5 The result of fluorescence standard curve
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Materials:
Competent cells (Escherichia coli strain DH5α)
LB (Luria Bertani) media
Chloramphenicol (stock concentration 25 mg/mL dissolved in EtOH)
50 ml Falcon tube (or equivalent, preferably amber or covered in foil to block light) Incubator at 37°C
1.5 ml eppendorf tubes for sample storage
Ice bucket with ice
Micropipettes and tips
96 well plate, black with clear flat bottom preferred
![](https://static.igem.org/mediawiki/2018/8/8a/T--Peking--interlabdevice.png)
![](https://static.igem.org/mediawiki/2018/5/55/T--Peking--devicelocation.png)
![](https://static.igem.org/mediawiki/2018/5/57/T--Peking--rawplatereadings1.png)
![](https://static.igem.org/mediawiki/2018/0/02/T--Peking--rawplatereadings2.png)
![](https://static.igem.org/mediawiki/2018/e/ec/T--Peking--fluorescence_per_od1.png)
![](https://static.igem.org/mediawiki/2018/6/69/T--Peking--fluorescence_per_od2.png)
![](https://static.igem.org/mediawiki/2018/7/7b/T--Peking--fluorescence_per_od3.png.png)
![](https://static.igem.org/mediawiki/2018/1/1a/T--Peking--fluorescence_per_particle1.png)
![](https://static.igem.org/mediawiki/2018/6/6d/T--Peking--fluorescence_per_particle2.png)
![](https://static.igem.org/mediawiki/2018/c/c4/T--Peking--fluorescence_per_particle3.png)
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(a) Colony 1 | (b) Colony 2 |
Figure. 11 The result of flow cytometry at 0h.
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(a) Colony 1 | (b) Colony 2 |
Figure. 12 The result of flow cytometry at 6h.
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![](https://static.igem.org/mediawiki/2018/8/8c/T--Peking--CFU.png)