Difference between revisions of "Team:Lethbridge/Improve"
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| − | <p class="f12">The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in <i>E. coli</i> and used for iGEM user applications. Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the <a href="https://2017.igem.org/Team:Lethbridge">Lethbridge iGEM team </a> in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing. </p> | + | <p class="f12">The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in <i>E. coli</i> and used for iGEM user applications. Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the <a href="https://2017.igem.org/Team:Lethbridge">Lethbridge iGEM team </a> in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing.</p> |
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| − | < | + | <h3 id="left"><a href="http://parts.igem.org/Part:BBa_K2683016" target="_blank" class="f12">Release Factor 1</a></h3> |
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| − | <h1><a href="http://parts.igem.org/Part:BBa_K2683017" target="_blank" class="f12>Release Factor 2</a></h1> | + | <h1><a href="http://parts.igem.org/Part:BBa_K2683017" target="_blank" class="f12">Release Factor 2</a></h1> |
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Revision as of 01:35, 18 October 2018
The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in E. coli and used for iGEM user applications. Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the Lethbridge iGEM team in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing.
- Original Part: BBa_K2443029
- Submitted by: Lethbridge iGEM 2017
- Designed by: iGEM17_Lethbridge
- Brief description: Release factor 1 (RF1) works with release factor 2 and 3 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.
- Original Part: BBa_K2443030
- Submitted by: Lethbridge iGEM 2017
- Designed by: iGEM17_Lethbridge
- Brief Description: Release factor 2 (RF2) works with release factors 1 and 2 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.