Difference between revisions of "Team:Lethbridge"
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| − | <p class=" | + | <p class="f12">VINCEnT is a standardized toolkit for simple design and assembly of custom protein nanocompartments (PNCs) for delivery of small molecules, nucleic acids, or proteins to specific cell targets. PNCs have user-specified surface proteins, encapsulation proteins, and cargo-loading approaches with wide-ranged applicability including targeted antibiotic delivery, rapid vaccine development, gene therapy, materials synthesis, improved cell culture transfection, specific biological control agents, and more. </p> |
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| − | <p class=" | + | <p class="f12">To enhance the accessibility of this toolkit, a software tool is in development that enables users to design a novel PNC to their own specifications based on suggested parts for their intended application. Standardized protocols will accompany the pre-designed toolkit parts and, together with the simplified design, less experienced users will be able to safely conduct complex experiments in a basic laboratory environment.</p> |
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| − | <p class="f18"> | + | <p class="f18">A risk assessment rubric was developed for our toolkit with advice from PHAC (Public Health Agency of Canada) and members of ELBI (Emerging Leaders in Biosecurity Initiative).</p> |
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| − | <li class=" | + | <li class="f14"> <b>Fifteen</b> parts improved from our <a href="https://2017.igem.org/Team:Lethbridge" target="_blank">N<i>ex</i>t <i>Vivo</i></a> project. </li> |
| − | <li class=" | + | <li class="f14"> Our goal with these parts was to continue standardizing, improving, and simplifying our protocols.</li> |
| − | <li class=" | + | <li class="f14"> We are now beginning design of Cell Free PNC Synthesis.</li> |
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Revision as of 02:21, 18 October 2018
Protein Nanocompartments
VINCEnT is a standardized toolkit for simple design and assembly of custom protein nanocompartments (PNCs) for delivery of small molecules, nucleic acids, or proteins to specific cell targets. PNCs have user-specified surface proteins, encapsulation proteins, and cargo-loading approaches with wide-ranged applicability including targeted antibiotic delivery, rapid vaccine development, gene therapy, materials synthesis, improved cell culture transfection, specific biological control agents, and more.
Software
To enhance the accessibility of this toolkit, a software tool is in development that enables users to design a novel PNC to their own specifications based on suggested parts for their intended application. Standardized protocols will accompany the pre-designed toolkit parts and, together with the simplified design, less experienced users will be able to safely conduct complex experiments in a basic laboratory environment.
Risk Assessment Rubric
A risk assessment rubric was developed for our toolkit with advice from PHAC (Public Health Agency of Canada) and members of ELBI (Emerging Leaders in Biosecurity Initiative).
Cell-free Synthesis Components
- Fifteen parts improved from our Next Vivo project.
- Our goal with these parts was to continue standardizing, improving, and simplifying our protocols.
- We are now beginning design of Cell Free PNC Synthesis.
