Difference between revisions of "Team:Lethbridge/Improve"

Revision as of 02:55, 18 October 2018

The following constructs are improved by being cloned and confirmed to be into the pSB1C3 plasmid, thus making them able to express in E. coli and used for iGEM user applications. Our project focuses on encapsulation of cargo by protein nanocompartments. This technology can be used to encapsulate cell free systems. The Next Vivo project, designed by the Lethbridge iGEM team in 2017 is therefore an important and viable application for our toolkit. We hope in the future to try encapsulating this system as an alternative to liposome encapsulation as previously proposed for this project. The ability to target cell types could increase the cell free technology we are still developing.

Release Factor 1

BBa_K2443029

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: Release factor 1 (RF1) works with release factor 2 and 3 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.

Release Factor 2

BBa_K2443030

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: Release factor 2 (RF2) works with release factors 1 and 2 to cause the termination of translation by recognizing the stop codon in the mRNA sequence in question.

Cysteine tRNA Synthetase

BBa_K2443004

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: Cysteine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Lysine tRNA Synthetase

BBa_K2443012

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: Lysine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha)

BBa_K2443014

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: Phenylalanine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis. The alpha subunit is one of the two subunits for this function.

Serine tRNA Synthetase (SerRS)

BBa_K2443019

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: Serine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Valine tRNA Synthetase (ValRS)

BBa_K2443023

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description:Valine tRNA synthetase works in the cell by attaching cysteine onto its tRNA to form an aminoacyl-tRNA for protein synthesis.

Initiation Factor I (IF1)

BBa_K2443024

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: IF1 is a prokaryotic protein that interacts with the ribosome by binding to the A site, preventing aminoacyl tRNA from entering the ribosome.

Initiation Factor 3 (IF1)

BBa_K2443025

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: IF-3 is a prokaryotic protein that binds to the 30S subunit of the ribosome to increase chances of availability for starting protein synthesis.

Elongation Factor- Thermo Unstable (EF-Tu)

BBa_K2443027

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description:EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor which catalyzes the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome for the creation of the peptide chain.

Elongation Factor-Thermo Stable (EF-Ts)

BBa_K2443028

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: EF-Ts is a prokaryotic elongation factor. EF-Ts acts at the guanine nucleotide exchange factor for EF-Tu. It releases GTP from EF-Tu, enabling EF-Tu to bind to a new GTP molecule and catalyze another tRNA addition.

MyoKinase (MK)

BBa_K2443033

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief Description: Myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP), which is crucial for energy homeostasis in the cell.

Nucleoside Diphosphate Kinase (NDK)

BBa_K2443035

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: NDK is responsible for the exchange of a phosphates between different nucleoside diphosphates (NDP) and triphosphates (NTP).

Peptidyl Prolyl Isomerase (PPiase)

BBa_K2443036

Submitted by: Lethbridge iGEM 2017
Designed by: iGEM17_Lethbridge
Brief description: This protein catalyzes the addition of the proline amino acid onto the peptide chain during protein synthesis.

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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683018" target="_blank" class="f12">Cysteine tRNA Synthetase</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683019" target="_blank" class="f12">Lysine tRNA Synthetase</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/d/d8/T--Lethbridge--Lys2018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683020" target="_blank" class="f12">Phenylalanine tRNA Synthetase alpha subunit (PheRS alpha) </a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/b/b6/T--Lethbridge--Phe2018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683022" target="_blank" class="f12">Serine tRNA Synthetase (SerRS) </a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/e/ee/T--Lethbridge--Ser2018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683023" target="_blank" class="f12">Valine tRNA Synthetase (ValRS) </a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/8/87/T--Lethbridge--Val2018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683024" target="_blank" class="f12">Initiation Factor I (IF1)</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/f/fb/T--Lethbridge--IF12018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683025" target="_blank" class="f12">Initiation Factor 3 (IF1)</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/f/f6/T--Lethbridge--IF32018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683026" target="_blank" class="f12">Elongation Factor- Thermo Unstable (EF-Tu) </a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/9/90/T--Lethbridge--Eftu.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683027" target="_blank" class="f12">Elongation Factor-Thermo Stable (EF-Ts)</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/1/12/T--Lethbridge--Efts.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683029" target="_blank" class="f12">Nucleoside Diphosphate Kinase (NDK)</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/0/0f/T--Lethbridge--NDK2018.png" /></center>
 </div></div>
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 <div class="textImage-Text"><h3><a href="http://parts.igem.org/Part:BBa_K2683030" target="_blank" class="f12">Peptidyl Prolyl Isomerase (PPiase)</a></h3></div>
-<div class="textImage-Image"><center><!--IMAGEGOESHERE--></center>
+<div class="textImage-Image"><center><img src="https://static.igem.org/mediawiki/2018/4/4d/T--Lethbridge--PPiase2018.png" /></center>
 </div></div>