Team:NCKU Tainan/Protocol
NCKU Tainan
Project
Description
Design
Improve
Results
Protocol
Measurement
Parts
Model
Model
Kinetic Law
Analysis
Device
Software
Hardware
Demonstrate
pH Meter
Thermometer
Wi-Fi Module
CO
2
Sensor
Safety
Human Practices
About
Integrated Human Practices
Education & Public Engagement
Product Design
Product design
Entrepreneurship
Team
Team Members
Collaborations
Attributions
Notebook
Awards
Accomplishment
Introduction
Device design
Bioreactor
Nutrient tank
pH Meter
Protocol
Arduino1
Arduino
test
20 μl system
50 μl system
Template
12～20 ng
30～50 ng
Forward primer
1.0 μl
2.5 μl
Reverse primer
1.0 μl
2.5 μl
dNTP
1.6 μl
4.0 μl
10x Buffer
2.0 μl
5.0 μl
Temperature
Time
94 ℃
3 min.
25～30 cycles
94 ℃(Denaturation)
40 sec
57.5 ℃(Annealing)
30 sec
72 ℃(Extension)
Depend on sequence size(2 kbp/min. for Taq)
72 ℃
5 min.
4 ℃
∞
Arduino
Arduino
