PCR Pasr and Pgada from E. coli MG1655 chromosome. Confirmed and extracted
products by DNA gel.
4/28
Digested the pSB1C3-sfGFP plasmid. Ligase the plasmid with Pasr / Pgada and
RiboJ by PCR. Confirmed by DNA gel electrophoresis. Transformed the gene in DH5
alpha and spread the bacteria on LB plates.
4/30~5/1
Confirmed the transformation by colony PCR ,enzyme digestion and DNA gel
electrophoresis. Selected the correct colonies for sequencing and cultured them
on LB plates.