We tried to get the bacteriocin from E. coli ER2566. To check whether the protein had been expressed, we used SDS-PAGE to confirm. Because our sequences content Intein and Chitin Binding Domain(CBD), which are 28 kDa, the result should be check as the bacteriocins’ initial mass plus 28 kDa. The figure showed our SDS-PAGE result. From here, we can know our target bacteriocin is produced.
Team:NCTU Formosa/Wet Lab/Expression
Protein Expression
Bacteriocin |
Mass(kDa) |
|---|---|
| Subtilocin | 5.8 |
| Enterocin A | 6.9 |
| Enterocin B | 7.5 |
| Enterocin 96 | 7.9 |
| Bovicin HJ50 | 6.25 |
| Durancin | 7.3 |
| Leucocyclicin Q | 6.4 |
| Lacticin Z | 5.9 |
Figure : SDS-PAGE result of the bacteriocin. (N1) Negative control: E. coli ER2566 without plasmid (N2) Negative control: E. coli ER2566 with empty plasmid (A) Enterocin 96+intein+CBD(35.9kDa) (B) Enteroicin B+intein+CBD(35.5kDa) (C) Leucocyclicin Q+intein+CBD(34.4kDa) (D) Durancin +intein+CBD(35.3kDa) (E) Lacticin+intein+CBD(33.9kDa) (F)Bovicin HJ50+intein +CBD(34.25kDa)
