Team:SZU-China/InterLab
InterLab Study
"Measurement is fundamentally an act of communication." - Jacob Beal
Reliable and repeatable measurement is a key component to all engineering disciplines. The same holds true for synthetic biology, which has also been called engineering biology. However, the ability to repeat measurements in different labs has been difficult. The Measurement Committee, through the InterLab study, has been developing a robust measurement procedure for green fluorescent protein (GFP) over the last several years. We chose GFP as the measurement marker for this study since it's one of the most used markers in synthetic biology and, as a result, most laboratories are equipped to measure this protein.
Calibration
OD600 Reference point
Measure the Abs600 of both the LUDOX CL-X (45% colloidal silica suspension) and water to obtain the correction factor.
Particle Standard Curve
Measure the Abs600 of a series of dilution monodisperse silica microspheres to draw the Particle Standard Curve.
Fluorescence standard curve
Measure the fluorescence of a series of diluted fluorescein to draw the fluorescein standard curve.
Cell measurement
We collaborate with the team SIAT-SCIE, because thire Distribution Kit was not delivered on time and we were during our final exam.
So we provide our kit and get the streaks of the transformed devices from them.
Colony Forming Units
