Team:Macquarie Australia/Design
The ultimate goal of our project was to produce vesicles in E. coli. We wanted to create a system that could enable simplified protein purification, as well as provide an isolated space for biochemistry within cells. During the first iteration of our design process, this general idea was our focus. After reviewing the literature, we realised that we could use the chlorophyll biosynthesis pathway to produce semi-crystalline aggregates of phospholipids and proteins called prolamellar bodies. When exposed to light, these spontaneously form vesicles. We saw this as a great opportunity to give chlorophyll an entirely new application within the biotechnology world. Ideally, our vesicles could be used in research labs, as well as on an industrial scale, in a broad range of projects. Before starting work in the lab, we needed to thoroughly design and plan our project, and to do this we established several guiding engineering principles. Example content number 1
Example content number 2
