Team:METU HS Ankara/Model
Modelling
Modeling in synthetic biology is a crucial tool that helps us to get a comprehensive vision of biological systems and the working principles while providing ways to improve the system. In our case, it significantly led to the design of our project while improving the part choices. Our project was based on the enhancement of fermentation by increasing the lifespan of an ethanologenic bacteria; E.coli strain KO11. Thus, we used simple models to simulate the kinetics of fermentation and enzymatic activities which provided insights on the formation of our experiments and the improvement of our project design.
What we have achieved
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We constructed our gene circuit with the help of toxicity analysis and estimation of enzymatic reactions by Michaelis and Menten enzyme kinetics.
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We used microbial growth and fermentation kinetics to simulate the expected behaviors of our system and the effects of our genes.
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We used microbial growth and fermentation kinetics to simulate the expected behaviors of our system and the effects of our genes.
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We improved the understanding of our project by demonstrating the pathways and effects of our genes.
Formation of Our Kinetic Models
Cell Growth Kinetic
The cell growth rate of bacteria depends on the number of cells and their specific growth rate. Thus, it can be expressed as: dX/dt = (µmax * X * S) / Ks + S