Team:Lethbridge/Human Practices
Interviews
Dr. Athanasios Zovoilis, University of Lethbridge
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Dr. Justin Pahara, Amino Bio Labs
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Dr. Vanessa Meier-Stephenson, Postdoctoral Fellow
Need a more effective tool for cell transfection
We spoke with Dr. Vanessa Meier-Stephenson, an MD-PhD working as a Postdoctoral Fellow in collaboration effort at the University of Lethbridge and the University of Calgary. During her PhD, she worked on drug design and told us that packaging methods for small molecules are designed to ensure that the drug can survive the acidic conditions of the stomach. Additionally, some larger drugs such as vancomycin cannot diffuse naturally across cell membranes. She suggested that for our protein nanocompartment system, it would be effective for this application by helping with moving larger molecules across membranes and for ensuring a smaller concentration of molecules would be required for certain cell types. In her own work, she said that there are difficulties in doing transfection assays using cell lines for Hepatitis B. She suggested that it would be useful if tags could be added to capsids to make them specific for different cell lines to increase the modularity of our system. Vanessa also suggested that we would need to look into toxicity levels in the cells and the potential immune response for safety considerations. Overall, this interview helped identify that there are issues with trying to transfect some cell lines, helped inform our construct design, and safety issues we may encounter for the drug delivery application.
Public Health Agency of Canada
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Nicole Kimmel, Alberta Environment and Parks
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Shannon Frank, Oldman Watershed Council
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Ryan Dyck, Agriculture and Agri-Food Canada
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Sweta Gupta, Pharmacist
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Regulatory Landscape
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| Regulation Area | Canada | United States |
|---|---|---|
| Environment | ||
| Food/Pharmaceuticals | ||
| Health | ||
| Laboratory Biosafety | ||
| Agriculture | ||
| Pests | ||
| Micro-organisms | ||
| Animals | ||
| Pathogens and Toxins |
Risk Assessment
What is Lorem Ipsum?
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.
Environmental Counter-Measures
What is Lorem Ipsum?
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.
Liability in iGEM
What is Lorem Ipsum?
Lorem Ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry's standard dummy text ever since the 1500s, when an unknown printer took a galley of type and scrambled it to make a type specimen book. It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages, and more recently with desktop publishing software like Aldus PageMaker including versions of Lorem Ipsum.