Team:METU HS Ankara/Experiments
Experiments
Materials:
- dH2O
- iGEM Kit Plates
- Pipette
Method:
- With a pipette tip, punch a hole through the foil cover into the corresponding well of the part desired.
- Pipette 10 µLof dH2O into the well. Pipette up and down several times and let sit for 5 minutes to make sure the dried DNA is fully resuspended. Resuspension will be in a crimson color, as the dried DNA has crisol dye.
- Transform resuspended DNA into an eppendorf tube.
Competent Cell Preparation Protocol:
Buffer 1:
- Potassium acetate 30 µL
- RbCl 100 µL
- CaCl 100 µL
- 87% glycerol 4,3 mL
- Complete to 25 mL
Buffer 2:
- MOPS 10 µL
- RbCl 10 µL
- CaCl 75 µL
- 87% glycerol 4,3 mL
- Complete to 25 mL
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