Basic Parts:
BBa_K2749017
xylE
The part codes for the enzyme catechol-2,3-dioxygenase which cleaves the oxidative ring of catechol to form 2-hydroxymuconate semialdehyde. It also contains a 6X His-tag which is a useful marker for purification and identification of the protein of interest.
Structure:
Fig1: 3D-Structure of enzyme catechol-2,3-dioxygenase (xylE)
Reaction Image taken from paper:
https://www.ingentaconnect.com/contentone/scs/chimia/2017/00000071/00000010/art00015?crawler=true&mimetype=application/pdf