Team:UC Davis/Notebook

iGEM

Notebook

Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14 Week 15 Week 16 Week 17 Week 18 Week 19

Lab Notebook

Week 1 (June 10-16)

Dry Lab Overview

Safety Training to work with Mammalian Cell Cultures. This week we read literature on Mammalian Biosensors to develop a frame work for our Project.

Week 2 (June 17-23)

Dry Lab and Outreach Overview

Before visiting the Yurok Tribe we did background research on the community and the area. This included not only scientific papers on the compounds in the area, but also the legislative and cultural context. We began to seriously consider how best to integrate human practices into the design of our project.

This week the team spent three days on the Yurok Reservation in Klamath, learning about the concerns of the tribe and the history of the land. We were able to make connections with the Superfund research core that has been working with the tribe and has collected data concerning the contamination in the Klamath river basin.

Week 3 (June 24-30)

Dry Lab Overview

We selected our reporter gene. After researching several options including luciferase, we selected EGFP. We acquired a plasmid already containing EGFP, which was coupled to the CMV strong constitutive promoter. We also made progress in choosing promoters that are related to the pathways we are interested in. We conducted research concerning physiological stress pathways. In particular, we studied literature published on the heat shock family of proteins. We began collecting FASTA files of our promoters of interest.

Week 4 (July 1-7)

Dry Lab Overview

We continued to do research on stress pathways and begin reading on mammalian cell culture and compiled papers to reference later in our protocols. We also continued to learn how to use the HTML language, in order to code our wiki.

Wet Lab Overview

To kickoff wet lab we began our InterLab study measurements. Our final data and findings are reproduced on our InterLab wiki. We followed the supplied iGEM interlab study protocol, available here: https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf We required several attempts to successfully transform the required DNA into our bacteria and obtained a new source of competent cells for use in our later experiments.

Week 5(July 8-14)

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