Team:Newcastle/Part Collection
Alternative Roots
Part Collection
We developed a collection of parts that enable an alternative measurement approach to both the InterLab study and assessing promoter ‘strength’ for other projects. Our parts collection includes an RFP device inserted into the pSB1C3 backbone that acts as an internal standard (IS) for the device being assessed. Four of the Interlab test devices have been returned with this modification. Second, we designed and deposited a new mNeonGreen fluorescent reporter part (BBa_K2797003) into the registry. This part demonstrated improved in the reproducibility over the existing GFPmut3b reporter currently in use in each test device. Five of the Interlab devices were returned with this modification.
New Interlab Parts Collection
| Name | Type | Description | Designer | Length(bp) |
|---|---|---|---|---|
| BBa_K2797003 | Basic | mNeonGreen fluorescent protein gene codon optimised for expression in E. coli | Kyle Stanforth | 711 |
| BBa_K2797004 | Composite | Test device 1 (originally BBa_J364000) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter | Kyle Stanforth | 909 |
| BBa_K2797005 | Composite | Test device 2 (originally BBa_J364001) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter | Kyle Stanforth | 909 |
| BBa_K2797006 | Composite | Test device 3 (originally BBa_J364002) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter | Kyle Stanforth | 909 |
| BBa_K2797007 | Composite | Test device 4 (originally BBa_J364007) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter | Kyle Stanforth | 909 |
| BBa_K2797008 | Composite | Positive control (originally BBa_I20270) of the iGEM 2018 InterLab study with mNeonGreen replacing the GFPmut3b fluorescent reporter | Kyle Stanforth | 910 |
| BBa_K2797013 | Plasmid vector | High copy BioBrick assembly plasmid pSB1C3 with RFP internal standard | Kyle Stanforth | 3073 |
References & Attributions
Attributions: Kyle Stanforth