We tried to express the six antimicrobial peptides in E. coli. These composite parts contains the coding sequence, RBS, a T7 promoter, and also intein and CBD sequences for better purification.
The other composite part contains each basic part from NCTU_Formosa 2016. We found this peptide also shows its ability to inhibit Bacillus subtilis.
Team:NCTU Formosa/Composite Part
Bacteriocins
Bovicin HJ50-Intein-CBD(BBa_K2599009)
Enterocin B-Intein-CBD(BBa_K2599011)
Enterocin 96-Intein-CBD(BBa_K2599012)
Lacticin Z-Intein-CBD(BBa_K2599013)
Leucocyclicin Q-Intein-CBD(BBa_K2599014)
Durancin TW-49M-Intein-CBD(BBa_K2599015)
Curcumin Bio-sensor
GS Linker-αS1-casein(BBa_K2599017)
Improvement
Sf1a+GS linker+snowdrop-lectin+linker+6X His-Tag(BBa_K2599016)
