The ideal medicine is not a perfect treatment . . .
. . . it's a cure
Advancements in genetic modification have opened avenues
towards numerous tracks of scientific
the demand for permanent health solutions has sparked massive interest in gene
Despite the potential for establishing an ideal medicine,
there are still numerous challenges.
The selection of an integration
methodology pertaining to gene
delivery, and maintenance of gene expression
currently limit the potential for gene
to become common practice.
How do we overcome these problems?
The system that allows for the integration and
maintenance of large-scale genetic constructs in eukaryotic systems.
University of Calgary’s Snip, Equip, Flip aims to overcome
the aforementioned obstacles. This system
allows for the creation of eukaryotic cell lines via stable integration and expression of exogenous
Snip, Equip, Flip is a simple and reliable method that can
be used by experienced and entrant
researchers alike and may facilitate a vast array of scientific discoveries.
By complementing the specificity of targeting that
CRISPR/Cas9 offers with the much larger integration
capabilities of FLP recombinase and beta resolvase, gene integration into eukaryotic genomes
simple process of Snip, Equip, Flip.
The expression of integrated genes is then protected and
maintained over time by adding flanking
chromatin modifying elements.