The chassis used were Acinetobacter Baylyi ADP1 and Ecoli DH5a which fall under the category of biosafety level 1 organisms. They are non-pathogenic bacteria which were genetically engineered in our project. These were handled with appropriate caution and care while following standard microbiological practices.+refer hereLaboratory Work Ethic and Design
The team underwent training for a period of two weeks to learn the appropriate safety measures and techniques needed to use the necessary laboratory equipment. All laboratory work was done wearing protective coats and gloves. The gloves were changed when they were contaminated and our hands were washed both after completion of work with hazardous material and before leaving the laboratory.
All laboratory waste was decontaminated in an autoclave prior to disposal. A safety shower and fire hydrants are located near the exit of the lab in case of emergencies.
- The laboratory supervisor must enforce the institutional policies that control access to the laboratory.
- Persons must wash their hands after working with potentially hazardous materials and before leaving the laboratory.
- Eating, drinking, smoking, handling contact lenses, applying cosmetics, and storing food for human consumption must not be permitted in laboratory areas. Food must be stored outside the laboratory area in cabinets or refrigerators designated and used for this purpose.
- Mouth pipetting is prohibited; mechanical pipetting devices must be used.
- Policies for the safe handling of sharps, such as needles, scalpels, pipettes, and broken glassware must be developed and implemented. Whenever practical, laboratory supervisors should adopt improved engineering and work practice controls that reduce risk of sharps injuries.Precautions, including those listed below, must always be taken with sharp items. These include:
- Careful management of needles and other sharps are of primary importance. Needles must not be bent, sheared, broken, recapped, removed from disposable syringes, or otherwise manipulated by hand before disposal.
- Used disposable needles and syringes must be carefully placed in conveniently located puncture-resistant containers used for sharps disposal.
- Non-disposable sharps must be placed in a hard walled container for transport to a processing area for decontamination, preferably by autoclaving.
- Broken glassware must not be handled directly. Instead, it must be removed using a brush and dustpan, tongs, or forceps. Plastic ware should be substituted for glassware whenever possible.
- Perform all procedures to minimize the creation of splashes and/or aerosols.
- Decontaminate work surfaces after completion of work and after any spill or splash of potentially infectious material with appropriate disinfectant.
- Decontaminate all cultures, stocks, and other potentially infectious materials before disposal using an effective method. Depending on where the decontamination will be performed, the following methods should be used prior to transport.
- Materials to be decontaminated outside of the immediate laboratory must be placed in a durable, leak proof container and secured for transport.
- Materials to be removed from the facility for decontamination must be packed in accordance with applicable government regulations.