Team:Pittsburgh/InterLab

InterLab Study

We decided to contribute to universalize the understanding of lab-to-lab variability of measurements by participating in the Interlab study. For the completion of the study, we followed the detailed InterLab protocol provided by the iGEM foundation and recorded the results.

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1. Calibration

1.1. OD600 Reference Point - LUDOX Protocol

Table 1

1.2. Particle Standard Curve - Microsphere Protocol

Table 2

Figure 1

Figure 2

Table 3

1.3. Fluorescence standard curve - Fluorescein Protocol

Table 4

Figure 3

Figure 4

Table 5

2.Cell Measurement

2.1. Plate Reader Raw Measurement

2.1.1. Fluorescence Raw Readings

Table 6

2.1.2. Abs600 Raw Readings

Table 7

2.2. Fluorescence per OD

Table 8

Table 9

Table 10

2.3. Fluorescence per Particle

Table 11

3. Colony Forming Units per 0.1 OD600 E.coli Cultures

Table 12

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