Team:Stuttgart/Basic Part
Basic Parts
A basic part is a functional unit of DNA that cannot be subdivided into smaller component parts.
| BioBrick | Type | Description | Designer | Length [bp] |
|---|---|---|---|---|
| BBa_K2848003 | Coding | pre-nisin A with internal YAAY- and His-tag | Lars Hoeing Liv Paul Luca Schelle |
207 |
| BBa_K2848002 | Coding | chitin deacetylase COD with C-terminal His-tag | Alexandra Mack Benjamin Schaefer |
1314 |
Our group managed to create two new basic parts which are both an improvement of already existing basic parts. BBa_K2848003 is an ORF for the lantibiotic Pre-Nisin A from Lactococcus lactis (BBa_K1365000) with two internal tags added. One is a conventional His6-Tag for easy purification via affinity chromatography. The second one is a tag of our own design, the YAAY-tag. This tag has the function to include tyrosines into nisin A which naturally has none. For our project this was of outmost importance when looking at the enzymatical linkage of nisin A to chitosan using a tyrosinase (see Experiments section).
BBa_K2848002 is the chitin deacetylase COD (BBa_K2380044) with a His-tag added C-terminally. This helps to purify the expressed proteins by affinity chromatography.