Team:VIT Vellore


VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced

Illustration 4

recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured