Difference between revisions of "Team:Jilin China/InterLab"

Bronze Medal Criterion #4

Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.

For teams participating in the InterLab study, all work must be shown on this page.

Introduction

Reliable and repeatable measurement remains one of the tough challenges in synthetic biology. In order to overcome this problem, the Measurement Committee, through the InterLab study, has been developing a measurement procedure for green fluorescent protein (GFP) for the last four years. The CFU count is a new feature of interlab study this year. The goal of the 2018 Interlab study is to determine how reliably known parts can conform to expectations and previous results and to calibrate OD¬ to colony forming unit (CFU) counts, which are directly relatable to the cell concentration of the culture, i.e. viable cell counts per mL. The CFU protocol assumes that 1 bacterial cell will give rise to 1 colony.

For a more detailed description visit the homepage of the iGEM's measurement committee.

Device We Received

Protocol

We strictly followed the protocol provided by iGEM. We measured the experiment of OD600 reference point and fitted the fluorescence standard curve first and then detected GFP expression in our E.coli with the plate reader Synergy HT from BioTek.

This is the transformation protocol.

This is the interlab plate reader protocol.

Raw Data

Table 1. The data of the OD600 reference point experiment

Table 2. Particle standard curve experiment

Table 3. The data of the Fluorescein standard curve experiment

Table 4. The data of the plate reader measurement experiment

Analysis

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Conclusion

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